FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l01n02_smallrna_ctrl025h_rep1_root.3520000006c1a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l01n02_smallrna_ctrl025h_rep1_root.3520000006c1a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17053322
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT365052521.406532991050074Illumina RNA PCR Primer (100% over 46bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG1561030.9153817655000005No Hit
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1558720.9140271907139267Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG1415430.8300025062565523No Hit
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT1005810.5898029721129994No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA909030.533051566140603Illumina RNA PCR Primer (100% over 45bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG873270.5120820447769648Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG786200.4610245440741692Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT782610.4589193823936474Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC748790.4390874692919069No Hit
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA679000.39816289166415786No Hit
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT651190.38185521859025473Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT609570.3574494166004723Illumina RNA PCR Primer (100% over 38bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG567710.3329028795679809Illumina RNA PCR Primer (100% over 34bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT554140.3249454856948107Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC530440.31104790022729883Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG509080.2985224814261995Illumina RNA PCR Primer (100% over 34bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT486470.285264067610991Illumina RNA PCR Primer (100% over 35bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT477750.2801506943925647Illumina RNA PCR Primer (100% over 38bp)
TATCTAGTATTCAGAGTTTGCCTCGATTTGGTACCGCGATCGTCGGACTGT436480.25595013100673286No Hit
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT424730.2490599778741057Illumina RNA PCR Primer (100% over 38bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC396050.2322421402703825Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC382000.22400327631179429Illumina RNA PCR Primer (100% over 39bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT342200.20066471506255495Illumina RNA PCR Primer (97% over 46bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT334460.19612600993519033Illumina RNA PCR Primer (100% over 35bp)
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC315830.18520145224490572No Hit
CCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT299050.1753617271755028Illumina RNA PCR Primer (100% over 31bp)
GCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT260610.1528206645016144Illumina RNA PCR Primer (100% over 38bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA259500.15216976492908538No Hit
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG256180.15022293017161115Illumina RNA PCR Primer (100% over 33bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT255470.14980658900359708Illumina RNA PCR Primer (100% over 35bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT254470.14922019299230965Illumina RNA PCR Primer (97% over 46bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT241210.14144458188263845Illumina RNA PCR Primer (100% over 38bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA235800.13827217946157352Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG235140.13788515809412383Illumina RNA PCR Primer (100% over 37bp)
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG228120.13376865809488617Illumina RNA PCR Primer (100% over 36bp)
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG219080.12846763815284787Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC210830.12362987105972666Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCAGATCGTCGGA204200.11974206550489108No Hit
GCCCGGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG202760.11889765524863717Illumina RNA PCR Primer (100% over 37bp)
GGCCGGCCGCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG191760.11244729912447556Illumina RNA PCR Primer (100% over 34bp)
GCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC190780.11187263103341391Illumina RNA PCR Primer (100% over 39bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT181210.1062608212053933Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG180390.10577997647613761Illumina RNA PCR Primer (100% over 34bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG179750.10540468302891366Illumina RNA PCR Primer (100% over 36bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG179050.10499420582101247Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGAGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG175400.1028538603798134Illumina RNA PCR Primer (100% over 36bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG175120.1026896694966529Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCGACGGGTTCGGGGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTG175090.10267207761631429Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG175000.10261930197529844Illumina RNA PCR Primer (100% over 37bp)
GCCCCCGTCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC172590.10120608758809574Illumina RNA PCR Primer (100% over 32bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG170650.10006847932619814Illumina RNA PCR Primer (100% over 36bp)
GCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG170540.10000397576495654Illumina RNA PCR Primer (100% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4015900.044.46432545
TGGCCTC483400.043.482481
ATAACCT52050.043.47719610
TAACCTA51750.042.9033411
CGATAAC53750.042.89748
GGGAATG194150.042.808452
CGCCGTA4201350.042.61792844
TCGCCGT4206250.042.53536643
GGTCGCC4218850.042.423141
GATAACC54450.042.3459179
TGGTCGC4222350.042.33210840
GTCGCCG4289550.041.81043642
GTGGTCG4286400.041.69925739
ACGATAA55150.041.6452837
TATCTAG119050.041.5401151
GACGATA55950.041.4920776
GGAATAC88900.041.3968286
GGCCTCG514700.041.293732
GGTTGCA23850.041.1298928
TCGGGCG523300.040.8073436
Produced by FastQC (version 0.11.2)