H3NGHBCXY_l01n01_smallrna_heat_1h_rep1_root.3510000006c252.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_heat_1h_rep1_root.3510000006c252.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9440577
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	3867947	40.97151053373115	RNA PCR Primer, Index 7 (100% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	81804	0.8665148327268555	RNA PCR Primer, Index 7 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	72684	0.7699105679663436	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	72307	0.7659171679866602	RNA PCR Primer, Index 7 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	52174	0.5526568979840957	RNA PCR Primer, Index 7 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	34126	0.36148214245802984	RNA PCR Primer, Index 7 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	28846	0.3055533575966808	RNA PCR Primer, Index 2 (100% over 34bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	28793	0.3049919512334892	RNA PCR Primer, Index 7 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	28353	0.30033121916171013	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGCTCATCTCGTATGCC	26326	0.2788600739128551	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACATCATCTCGTATGCC	21856	0.23151127309273575	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAAGATCATCTCGTATGCC	21170	0.22424476808991656	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAGCATCTCGTATGCC	20421	0.2163109309950017	RNA PCR Primer, Index 7 (98% over 51bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	19177	0.20313377031933535	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAACATCTCGTATGCC	19076	0.20206392045740423	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGATCATCTCGTATGCC	18514	0.1961108944929955	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATGATCTCGTATGCC	17363	0.183918843096137	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATTATCTCGTATGCC	15733	0.1666529492847736	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAATCATCTCGTATGCC	15010	0.15899451908500933	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGACCATCTCGTATGCC	14811	0.1568865970798183	RNA PCR Primer, Index 7 (98% over 51bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	12741	0.1349599711966758	RNA PCR Primer, Index 7 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGTTCATCTCGTATGCC	12618	0.13365708473115573	RNA PCR Primer, Index 7 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTGATCATCTCGTATGCC	11400	0.12075533095063998	RNA PCR Primer, Index 7 (98% over 51bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	11383	0.12057525721150307	RNA PCR Primer, Index 7 (100% over 39bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC	10242	0.10848913154354867	RNA PCR Primer, Index 7 (100% over 39bp)
TGGAAGTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC	10190	0.1079383177532475	RNA PCR Primer, Index 7 (98% over 51bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA	10102	0.10700617133889168	RNA PCR Primer, Index 7 (100% over 40bp)
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT	9742	0.10319284509834517	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	530560	44.140373	45
TTATCTC	4660	43.83042	38
AGTTCTC	12275	43.802658	4
TCTCGTA	533060	43.75605	41
GTTCTCG	14240	43.75544	5
ATCTCGT	533260	43.742043	40
CGTATGC	534480	43.72524	44
TCGTATG	535420	43.674942	43
CTCGTAT	535070	43.67324	42
CATCTCG	521850	43.66086	39
GGGATTC	9395	43.464863	2
TCATCTC	509555	43.407646	38
GGTATTC	6210	43.257515	2
GATTCTC	12355	43.24544	4
CAATTCT	11970	43.149124	3
TGCAATT	10250	43.123646	1
GCAATTC	10865	43.018562	2
TATTCTC	8110	42.985405	4
GGATTCT	10915	42.984165	3
GCATCTC	5320	42.95936	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content