H3NGHBCXY_l01n01_smallrna_ctrl1h_rep3_root.3510000006c238.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_ctrl1h_rep3_root.3510000006c238.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6270489
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	3354276	53.493052934149155	RNA PCR Primer, Index 3 (100% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	67774	1.0808407446373003	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	52685	0.840205604379499	RNA PCR Primer, Index 3 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	50494	0.805264150850117	RNA PCR Primer, Index 3 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	43816	0.6987652797094454	RNA PCR Primer, Index 3 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	43202	0.6889733799070535	RNA PCR Primer, Index 3 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	40104	0.6395673447477541	RNA PCR Primer, Index 3 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	26114	0.41645874827306134	RNA PCR Primer, Index 3 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	24407	0.38923599100484824	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGGCATCTCGTATGCC	20637	0.3291130883093807	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAAGCATCTCGTATGCC	20378	0.3249826289464825	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTCGGCATCTCGTATGCC	20257	0.3230529548811903	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTATGCATCTCGTATGCC	20220	0.3224628892579191	RNA PCR Primer, Index 3 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	18046	0.28779254696085105	RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTACGCATCTCGTATGCC	17223	0.27466757377295453	RNA PCR Primer, Index 3 (98% over 51bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC	15257	0.24331435714184335	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGTCATCTCGTATGCC	14796	0.23596245843027552	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGACATCTCGTATGCC	13961	0.2226461126078046	RNA PCR Primer, Index 3 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13566	0.2163467633863962	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGCCATCTCGTATGCC	13332	0.21261499701219475	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGGCATCTCGTATGCC	11052	0.17625419644305254	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAGGCATCTCGTATGCC	10645	0.16976347458706967	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAGGCATCTCGTATGCC	10024	0.1598599407478428	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGTATCTCGTATGCC	9148	0.1458897384239092	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGGATCTCGTATGCC	9027	0.14396006435861702	RNA PCR Primer, Index 3 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAAGGCATCTCGTATGCC	8088	0.12898515570316765	RNA PCR Primer, Index 3 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTATT	30	2.126866E-6	45.12254	1
TGCGTTA	40	6.8394E-9	44.988693	7
ATGCATC	3775	0.0	44.51164	36
GTTCTCG	8700	0.0	44.49438	5
TGCATCT	4245	0.0	44.458435	37
TATGCAT	2860	0.0	44.359127	35
GGGATTC	5475	0.0	44.326206	2
AGTTCTC	7905	0.0	44.323063	4
GTATGCC	443965	0.0	44.25118	45
CGAATTC	6690	0.0	44.123924	2
TGGAGTT	6320	0.0	44.12299	1
GGTATTC	3740	0.0	44.10303	2
TCTCGTA	445080	0.0	43.99753	41
TCGAATT	6270	0.0	43.97109	1
ATCTCGT	445285	0.0	43.97102	40
CATCTCG	438610	0.0	43.963005	39
AGCCATC	2170	0.0	43.951736	36
CGTATGC	446475	0.0	43.926422	44
CTCGTAT	446555	0.0	43.923237	42
TCGTATG	446960	0.0	43.91212	43

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content