H3NGHBCXY_l01n01_smallrna_ctrl1h_rep2_root.3510000006c22b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_ctrl1h_rep2_root.3510000006c22b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11322459
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	5929000	52.364950051927764	RNA PCR Primer, Index 1 (100% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	95613	0.8444543716166251	RNA PCR Primer, Index 1 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	86017	0.7597024639259017	RNA PCR Primer, Index 1 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	83723	0.7394418473937509	RNA PCR Primer, Index 1 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	77225	0.6820514872255222	RNA PCR Primer, Index 1 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	73267	0.6470944165044007	RNA PCR Primer, Index 1 (98% over 51bp)
TGGTATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	58424	0.5160009852983349	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACCATCTCGTATGCC	48794	0.4309487894811542	RNA PCR Primer, Index 1 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	43934	0.3880252514051939	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCCCGATCTCGTATGCC	43477	0.3839890257054585	RNA PCR Primer, Index 1 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	42898	0.3788752955519645	RNA PCR Primer, Index 1 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	32116	0.28364863145011165	RNA PCR Primer, Index 1 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAACACGATCTCGTATGCC	30010	0.26504843161719555	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATAACGATCTCGTATGCC	29404	0.25969623736327946	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCACGATCTCGTATGCC	28202	0.24908016889264073	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCACGATCTCGTATGCC	26328	0.23252899392260992	RNA PCR Primer, Index 1 (98% over 51bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC	26090	0.23042697703740855	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAAGATCTCGTATGCC	19722	0.17418477735269344	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCTCGATCTCGTATGCC	19057	0.16831149487933672	RNA PCR Primer, Index 1 (98% over 51bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG	16126	0.1424248919779705	RNA PCR Primer, Index 1 (100% over 39bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACATCACGATCTCGTATGCCG	16110	0.1422835799184612	RNA PCR Primer, Index 1 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGACGATCTCGTATGCC	13595	0.12007109056433765	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACATCACGATCTCGTATGCCG	13188	0.11647646505056897	TruSeq Adapter, Index 1 (96% over 33bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	13108	0.11576990475302229	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACTATCTCGTATGCC	12504	0.11043537450654492	RNA PCR Primer, Index 1 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGACTCCAGTCACATCACGATCTCGTATGCCG	12236	0.10806839750976358	Illumina PCR Primer Index 1 (96% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATATCGTATGCC	11483	0.1014178987091055	RNA PCR Primer, Index 1 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATTC	7395	44.152073	2
AGTTCTC	14900	44.125847	4
GGGATTC	10520	44.12375	2
GTTCTCG	16710	44.11953	5
CTATCTC	3855	44.055943	38
GTATGCC	781500	44.011917	45
TGCAATT	14275	43.988834	1
GATTCTC	13380	43.985657	4
CAATTCT	16590	43.976887	3
CCATCTC	10630	43.97382	38
TCTCGTA	778665	43.906055	41
ATCTCGT	778990	43.87811	40
GATCTCG	760855	43.871517	39
TGGAGTT	11915	43.858105	1
CGTATGC	783860	43.799026	44
TCGAATT	10185	43.79767	1
CTCGTAT	782500	43.795807	42
TCGTATG	784945	43.779755	43
GCAATTC	15230	43.77902	2
CGATCTC	753290	43.752693	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content