H3NGHBCXY_l01n01_smallrna_ctrl1h_rep1_root.3510000006c201.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_ctrl1h_rep1_root.3510000006c201.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8726571
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	4106961	47.06271226120775	RNA PCR Primer, Index 11 (100% over 51bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	80506	0.9225387612156024	RNA PCR Primer, Index 11 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	69957	0.8016550830790238	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	64574	0.7399699148726344	RNA PCR Primer, Index 11 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	52933	0.6065727305719508	RNA PCR Primer, Index 11 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	52931	0.6065498120624928	RNA PCR Primer, Index 11 (98% over 51bp)
TGGATTTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	49639	0.5688259454945133	RNA PCR Primer, Index 11 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	42198	0.48355763105577204	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTACATCTCGTATGCC	37817	0.4333546360878746	RNA PCR Primer, Index 11 (98% over 51bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	32037	0.3671201437540587	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACTACATCTCGTATGCC	31895	0.36549292958253593	RNA PCR Primer, Index 11 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	29299	0.3357447043059639	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCTACATCTCGTATGCC	28882	0.3309661950839568	RNA PCR Primer, Index 11 (98% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	21982	0.25189733745362297	RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGCTACATCTCGTATGCC	19377	0.22204597888448968	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTCCATCTCGTATGCC	19226	0.22031563142040556	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATACATCTCGTATGCC	18055	0.20689684413270687	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCTACATCTCGTATGCC	17578	0.2014307796269577	RNA PCR Primer, Index 11 (98% over 51bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	17460	0.2000785875689317	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAGATCTCGTATGCC	16432	0.18829847370748487	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCCACATCTCGTATGCC	15827	0.18136562459641936	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTGCATCTCGTATGCC	13337	0.15283208032112497	RNA PCR Primer, Index 11 (98% over 51bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	12509	0.14334381740548494	RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACGGCTACATCTCGTATGCCG	12021	0.1377517010977164	RNA PCR Primer, Index 11 (97% over 36bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAC	11515	0.13195331820482525	RNA PCR Primer, Index 11 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCAACATCTCGTATGCC	10976	0.125776779905876	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAAGTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC	10700	0.12261402560066262	RNA PCR Primer, Index 11 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGAACTCCAGTCACGGCTACATCTCGTATGCCG	10520	0.12055135974943652	TruSeq Adapter, Index 11 (96% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTAATCTCGTATGCCG	9800	0.1123006963445321	RNA PCR Primer, Index 11 (97% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTATATCTCGTATGCC	8916	0.10217071516406616	RNA PCR Primer, Index 11 (98% over 51bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATTC	4690	44.15616	2
TGCATCT	3535	44.033756	37
GTTCTCG	11445	44.023006	5
GTATGCC	550805	43.94455	45
AGTTCTC	10215	43.875305	4
TCGAATT	9110	43.765617	1
GGGATTC	7005	43.750134	2
CATCTCG	542605	43.72932	39
TCTCGTA	552020	43.716606	41
ATCTCGT	552475	43.684982	40
ATACATC	3425	43.674725	36
TGCAATT	10155	43.639164	1
CTCGTAT	553940	43.627213	42
CGTATGC	553865	43.627026	44
TCGTATG	554590	43.602848	43
TGGAGTT	8005	43.577576	1
TGGTATT	4440	43.552467	1
CATTCCT	2465	43.528435	16
CTGCATC	2595	43.51465	36
ACATCTC	535265	43.505043	38

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content