FastQCFastQC Report
Thu 27 Oct 2016
H3NGHBCXY_l01n01_smallrna_ctrl025h_rep3_root.3510000006c1e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NGHBCXY_l01n01_smallrna_ctrl025h_rep3_root.3510000006c1e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11120743
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC195725117.600002086191544RNA PCR Primer, Index 10 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1702051.5305182396535915RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT617520.5552866386715348RNA PCR Primer, Index 10 (100% over 39bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA565760.5087429859677541RNA PCR Primer, Index 10 (100% over 40bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT565350.5083743055657343RNA PCR Primer, Index 3 (100% over 34bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA442640.39803095890265605RNA PCR Primer, Index 10 (100% over 40bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG398430.358276420918998No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA337400.3033969942475966No Hit
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC322340.2898547336270607RNA PCR Primer, Index 10 (98% over 51bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT318270.2861949062216436RNA PCR Primer, Index 10 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTCATCTCGTATGCC309040.2778951010737322RNA PCR Primer, Index 10 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC267380.24043357534653934RNA PCR Primer, Index 10 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC250790.2255155073721243RNA PCR Primer, Index 10 (98% over 51bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT248190.2231775340910225No Hit
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT245180.2204708804079008RNA PCR Primer, Index 10 (100% over 39bp)
GCCGTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG239990.2158039260506245RNA PCR Primer, Index 7 (97% over 36bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT223940.20137143714228445RNA PCR Primer, Index 1 (100% over 30bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA213860.1923072945755513RNA PCR Primer, Index 1 (100% over 32bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA205220.18453802951835144RNA PCR Primer, Index 7 (97% over 35bp)
GCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC204880.1842322945508227RNA PCR Primer, Index 10 (100% over 42bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT199790.17965526224281958RNA PCR Primer, Index 10 (100% over 39bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT188890.16985375887204657RNA PCR Primer, Index 10 (100% over 43bp)
CCGACCTGCTCGACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT174580.15698591362105932RNA PCR Primer, Index 3 (100% over 34bp)
GCCCCCGACGTCGCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA170410.1532361641663691RNA PCR Primer, Index 7 (97% over 35bp)
GGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT167530.15064640914730248RNA PCR Primer, Index 10 (100% over 41bp)
GGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT163070.14663588574972014RNA PCR Primer, Index 10 (100% over 41bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC160530.14435186569818223RNA PCR Primer, Index 1 (100% over 33bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA160440.14427093585383638No Hit
GCCCCCGACGTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC158830.1428231908605387RNA PCR Primer, Index 10 (100% over 37bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC155740.1400445995379985RNA PCR Primer, Index 10 (100% over 42bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT154530.13895654274179342RNA PCR Primer, Index 10 (100% over 41bp)
GCCGCGGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT152870.13746383672385917RNA PCR Primer, Index 10 (100% over 38bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT151530.13625888126359903RNA PCR Primer, Index 10 (100% over 39bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA150910.13570136455810553RNA PCR Primer, Index 7 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCGTATCTCGTATGCC149330.1342805961795898RNA PCR Primer, Index 10 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC146560.13178975541472362RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCCTATCTCGTATGCC145370.1307196830283732RNA PCR Primer, Index 10 (98% over 51bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC142290.1279500839107603No Hit
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT140090.12597179882675105RNA PCR Primer, Index 10 (100% over 39bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC137990.12408343579201497RNA PCR Primer, Index 10 (98% over 51bp)
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA136040.12232995583118862RNA PCR Primer, Index 10 (100% over 40bp)
GCCCCCGACGTCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG134880.12128686005962012RNA PCR Primer, Index 7 (97% over 36bp)
GGGGGACGGACCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG128820.11583758387366744RNA PCR Primer, Index 7 (97% over 36bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC124760.11218674867317768No Hit
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT121250.10903048474369025RNA PCR Primer, Index 10 (100% over 39bp)
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATT116840.10506492237074448No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTGATCTCGTATGCC116040.1043455459765593RNA PCR Primer, Index 10 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCATATCTCGTATGCC115320.10369810722179265RNA PCR Primer, Index 10 (98% over 51bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT114210.10269997247486073RNA PCR Primer, Index 10 (100% over 43bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGCTTATCTCGTATGCC112510.10117129763721723RNA PCR Primer, Index 10 (98% over 51bp)
GGGCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC111840.10056881990708713RNA PCR Primer, Index 10 (100% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCC285600.044.35162714
GTATGCC2639500.044.0346145
GTATAGT75900.043.8337510
CGTATGC2658700.043.6472144
CACACGA46250.043.554431
TCGTATG2669600.043.50775543
CTCGTAT2671200.043.46906742
TATAGTG76700.043.43520711
TCTCGTA2671800.043.40120741
TAGTATA78550.043.2713558
ATCTCGT2680900.043.26292840
TATTCCC76600.043.1101523
GTGAGTA76950.042.94329518
AGTATTC77400.042.75175521
TTCCCGC77100.042.71386725
GAGTATT77250.042.6891720
CGTTGTA70200.042.6836133
TGTAGTA77100.042.658786
AGTATAG78800.042.5631379
GTAGTAT79500.042.471327
Produced by FastQC (version 0.11.2)