H3NGHBCXY_l01n01_smallrna_ctrl025h_rep2_root.3510000006c1cc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_ctrl025h_rep2_root.3510000006c1cc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8842507
Sequences flagged as poor quality	0
Sequence length	51
%GC	54

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	2647109	29.936182125725203	RNA PCR Primer, Index 9 (100% over 51bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	51425	0.581565838737815	RNA PCR Primer, Index 6 (100% over 34bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	46723	0.5283908737646461	RNA PCR Primer, Index 9 (98% over 51bp)
TGGGATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	36737	0.41545910000410513	RNA PCR Primer, Index 9 (98% over 51bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	36176	0.40911474539969267	RNA PCR Primer, Index 9 (98% over 51bp)
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	33009	0.37329911076123545	RNA PCR Primer, Index 9 (98% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	31547	0.35676533815579675	RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	30792	0.34822703561331647	RNA PCR Primer, Index 9 (100% over 39bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA	28895	0.3267738436622103	RNA PCR Primer, Index 9 (100% over 40bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA	26478	0.29943996651628324	RNA PCR Primer, Index 9 (100% over 40bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	24388	0.2758041356370993	RNA PCR Primer, Index 9 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACCAGATCTCGTATGCC	23434	0.265015340106601	RNA PCR Primer, Index 9 (98% over 51bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	22682	0.2565109645940908	RNA PCR Primer, Index 7 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCACATCTCGTATGCC	22529	0.2547806860656146	RNA PCR Primer, Index 9 (98% over 51bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	19914	0.22520762494165963	RNA PCR Primer, Index 9 (98% over 51bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	18904	0.2137855248517191	RNA PCR Primer, Index 9 (100% over 43bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT	18511	0.20934108392563333	RNA PCR Primer, Index 9 (100% over 41bp)
TAGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	18072	0.20437642854000568	RNA PCR Primer, Index 9 (98% over 51bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC	17934	0.20281578516138013	RNA PCR Primer, Index 9 (100% over 42bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAGCAGATCTCGTATGCC	14189	0.16046354274867974	RNA PCR Primer, Index 9 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	13607	0.15388169893447637	RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCCGATCTCGTATGCC	13489	0.15254723575565166	RNA PCR Primer, Index 9 (98% over 51bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	13183	0.14908667869869935	RNA PCR Primer, Index 9 (100% over 39bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	13037	0.1474355632401535	No Hit
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT	12559	0.1420298564649143	RNA PCR Primer, Index 9 (100% over 43bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG	12321	0.13933831208728475	RNA PCR Primer, Index 9 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATAAGATCTCGTATGCC	12218	0.13817348405831062	RNA PCR Primer, Index 9 (98% over 51bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	12074	0.13654498661974485	RNA PCR Primer, Index 7 (97% over 35bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC	11981	0.13549324869067111	RNA PCR Primer, Index 9 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTCAGATCTCGTATGCC	11375	0.12863998863670675	RNA PCR Primer, Index 9 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAACAGATCTCGTATGCC	10897	0.12323428186146757	RNA PCR Primer, Index 9 (98% over 51bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA	10636	0.12028263025406709	RNA PCR Primer, Index 9 (100% over 38bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTCAGATCTCGTATGCC	10494	0.11867675083548138	RNA PCR Primer, Index 9 (98% over 51bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10393	0.11753454082648733	RNA PCR Primer, Index 1 (100% over 32bp)
GGCGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC	9430	0.10664396420607866	RNA PCR Primer, Index 9 (100% over 42bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	349395	44.35726	45
CGTATGC	351095	44.0534	44
CTCGTAT	351240	44.00511	42
TCTCGTA	350345	43.99296	41
TCGTATG	352130	43.962246	43
ATCTCGT	351555	43.84169	40
GATCTCG	344930	43.533855	39
CATTCCC	5530	42.99609	14
CACATCT	4535	42.95559	37
GTTCTCG	7890	42.888706	5
AGTTCTC	6970	42.796204	4
GGGATTC	4750	42.736206	2
AGATCTC	345555	42.60248	38
TGTAGTA	2725	42.59849	6
TAGTATA	2830	42.525448	8
CGTTGTA	5300	42.253292	3
GCAATTC	6665	42.187054	2
TGGAGTT	5660	42.171333	1
GATTCTC	6135	42.047573	4
CACACGA	3020	41.983387	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content