H3NGHBCXY_l01n01_smallrna_ctrl025h_rep1_root.3510000006c1a3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NGHBCXY_l01n01_smallrna_ctrl025h_rep1_root.3510000006c1a3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17053322
Sequences flagged as poor quality	0
Sequence length	51
%GC	55

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	3161677	18.539947817791745	RNA PCR Primer, Index 2 (100% over 51bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT	133401	0.7822581430175305	No Hit
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC	123225	0.7225864849089227	No Hit
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	118323	0.6938413524356134	RNA PCR Primer, Index 1 (100% over 30bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG	70896	0.41573131616232895	No Hit
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC	67098	0.3934599956536328	RNA PCR Primer, Index 2 (100% over 34bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	66312	0.38885092300491364	RNA PCR Primer, Index 2 (100% over 39bp)
TGCAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	60650	0.3556491808458199	RNA PCR Primer, Index 2 (98% over 51bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	55260	0.32404243583742803	RNA PCR Primer, Index 2 (100% over 39bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	55244	0.32394861247562207	RNA PCR Primer, Index 2 (100% over 40bp)
TGGAGTTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	53302	0.3125608019364204	RNA PCR Primer, Index 2 (98% over 51bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT	50207	0.2944118453870747	No Hit
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	45028	0.26404239596249923	RNA PCR Primer, Index 2 (98% over 51bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA	42194	0.24742393300261378	No Hit
TGAAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	40111	0.23520930408749682	RNA PCR Primer, Index 2 (98% over 51bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	39091	0.22922806477236518	RNA PCR Primer, Index 2 (100% over 43bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	37645	0.2207487784491491	RNA PCR Primer, Index 2 (100% over 39bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	37548	0.2201799743182003	RNA PCR Primer, Index 1 (100% over 30bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	36705	0.21523665594304736	RNA PCR Primer, Index 2 (100% over 40bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	36362	0.21322531762433147	RNA PCR Primer, Index 2 (100% over 39bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGCATCTCGTATGCC	35647	0.20903258614362644	RNA PCR Primer, Index 2 (98% over 51bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	34881	0.20454079269716482	RNA PCR Primer, Index 2 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGCTGTATCTCGTATGCC	33324	0.1954106068014197	RNA PCR Primer, Index 2 (98% over 51bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	30435	0.17846962603532615	RNA PCR Primer, Index 2 (100% over 43bp)
GCGGTACCAAATCGAGGCAAACTCTGAATACTAGATATGGAATTCTCGGGT	29996	0.17589534754577438	No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	25097	0.14716780695280368	RNA PCR Primer, Index 2 (100% over 35bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT	24907	0.1460536545313576	RNA PCR Primer, Index 2 (100% over 43bp)
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA	24236	0.142118937295619	RNA PCR Primer, Index 2 (100% over 40bp)
CGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC	23174	0.1358914116557466	RNA PCR Primer, Index 2 (98% over 51bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAAGTATCTCGTATGCC	23115	0.13554543800908703	RNA PCR Primer, Index 2 (98% over 51bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC	23030	0.13504700139949272	RNA PCR Primer, Index 2 (100% over 44bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA	22171	0.13000985966253378	No Hit
GGGGGACGGACCGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA	20377	0.11948991522003749	RNA PCR Primer, Index 2 (100% over 36bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG	20361	0.1193960918582315	RNA PCR Primer, Index 2 (100% over 38bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG	18475	0.10833666308535075	RNA PCR Primer, Index 2 (100% over 35bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT	17760	0.10414393160464572	RNA PCR Primer, Index 2 (100% over 39bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC	17654	0.10352235183268106	No Hit
GGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTAT	17600	0.10320569798658583	RNA PCR Primer, Index 2 (100% over 41bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGACGTATCTCGTATGCC	17542	0.10286558830003914	RNA PCR Primer, Index 2 (98% over 51bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	17418	0.10213845724604273	RNA PCR Primer, Index 1 (100% over 33bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC	17278	0.10131750283024034	RNA PCR Primer, Index 2 (100% over 42bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCCG	17197	0.10084252206109753	RNA PCR Primer, Index 2 (98% over 51bp)
TGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCTC	17104	0.10029717377060025	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTATGCC	424655	43.83232	45
CATTCCC	19690	43.504505	14
CGTATGC	427335	43.470688	44
TCTCGTA	426650	43.458378	41
CTCGTAT	427805	43.429913	42
TCGTATG	428860	43.34838	43
ATCTCGT	428415	43.2892	40
CACACGA	6425	43.182316	1
CATTCCT	7360	43.00305	16
TAGTATA	9280	43.002125	8
GTATAGT	9240	42.896137	10
AGTATAG	9270	42.73305	9
TATAGTG	9290	42.665268	11
TGCATCT	6685	42.566906	37
GCATCTC	8360	42.46136	38
GAGTATT	9165	42.167095	20
GTAGTAT	9390	42.139027	7
TATTCCC	9235	42.066696	23
TGTAGTA	9235	41.972343	6
CATCTCG	10310	41.91427	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content