FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l02n02_smallrna_heat_1h_rep3_shoot.3520000006c000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l02n02_smallrna_heat_1h_rep3_shoot.3520000006c000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101275
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT13271.3102937546284867Illumina RNA PCR Primer (100% over 46bp)
TGGCGGGACTGACGGGGCTCGAACCCGCAGCTTCCGCCTTGACAGGGCGGT2630.25968896568748456No Hit
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1620.15996050357936312Illumina DpnII expression Sequencing Primer (96% over 30bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGAT207.002816E-445.00000421
GCCGTAT1450.045.00000445
GGGGAAT207.002816E-445.0000041
CTCGAAC253.8655715E-545.018
GCGGGAC253.8655715E-545.03
AGCTTCC253.8655715E-545.029
CGCCTTG253.8655715E-545.035
GGACTGA253.8655715E-545.06
GACGGGG253.8655715E-545.011
AGGGCGG253.8655715E-545.044
TGACAGG253.8655715E-545.040
AACCCGC253.8655715E-545.022
ACCCGCA253.8655715E-545.023
CCCGCAG253.8655715E-545.024
TCGCCGT1500.045.043
GAACCCG351.1973134E-745.021
GCTCGAA253.8655715E-545.017
GGCTCGA253.8655715E-545.016
ACGGGGC253.8655715E-545.012
GACTGAC253.8655715E-545.07
Produced by FastQC (version 0.11.2)