H3NFYBCXY_l02n02_smallrna_heat_1h_rep1_shoot.3520000006bfe8.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l02n02_smallrna_heat_1h_rep1_shoot.3520000006bfe8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32110607
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	2355124	7.334411336416033	Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	797317	2.483033098689165	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	383997	1.1958571820208816	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	297187	0.9255103773030513	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	179658	0.5594973648427138	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	177028	0.5513069248426229	Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	156696	0.48798828374686287	Illumina DpnII expression Sequencing Primer (100% over 24bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	151174	0.4707914739824134	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	146304	0.4556251459214085	Illumina RNA PCR Primer (100% over 34bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	143010	0.4453668533889752	Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	136750	0.42587173764731384	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	134747	0.4196339234571305	Illumina RNA PCR Primer (100% over 34bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	127848	0.3981488110766639	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	126941	0.39532419925914203	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	118027	0.36756390185959426	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	116837	0.36385796132723375	Illumina RNA PCR Primer (97% over 45bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	116215	0.3619209066960335	Illumina RNA PCR Primer (100% over 45bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	114917	0.3578786287035932	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	106674	0.33220798348657815	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	104777	0.3263002782849916	No Hit
TCAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	98743	0.30750897982090464	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	98428	0.3065279955623387	Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	98311	0.30616362998058555	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	95060	0.29603924958503586	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	94313	0.29371291548615075	Illumina RNA PCR Primer (100% over 38bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	87403	0.27219354651252775	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	86215	0.2684938344516502	Illumina RNA PCR Primer (100% over 35bp)
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	85032	0.26480969356948003	Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	79412	0.24730768870236552	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTGAGCTATAGCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	77653	0.24182974803310323	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	77181	0.2403598287631249	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	74922	0.23332477022312287	No Hit
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	73669	0.2294226328390491	Illumina RNA PCR Primer (100% over 34bp)
TCAGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAG	72285	0.22511253057284153	No Hit
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	66092	0.20582606862585934	Illumina RNA PCR Primer (100% over 35bp)
CAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGTG	63360	0.19731797658013753	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	62445	0.19446845087668382	No Hit
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	60091	0.18713753994124122	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	58411	0.18190562389555576	Illumina RNA PCR Primer (100% over 36bp)
CTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAA	55075	0.17151653346198031	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	54422	0.16948293752279425	Illumina RNA PCR Primer (100% over 34bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	54050	0.1683244418269639	Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTGATCGTCGGACTGTAGAACTCT	52059	0.16212399846567835	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	51218	0.1595049262070941	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	51187	0.15940838489910825	No Hit
AGTCCCGCCTGACCTGGGGTCGCGATCGTCGGACTGTAGAACTCTGAACGT	50990	0.1587948804580368	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG	50573	0.1574962441538399	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	49733	0.15488028613099714	Illumina RNA PCR Primer (100% over 33bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	49676	0.15470277469373284	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATGCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAG	49497	0.154145326496008	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	45637	0.14212437653389737	Illumina RNA PCR Primer (100% over 34bp)
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	44282	0.1379045871041927	Illumina RNA PCR Primer (100% over 35bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	44184	0.1375993920015277	Illumina RNA PCR Primer (100% over 37bp)
ATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCT	43847	0.13654989455664915	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	43491	0.13544122663268246	Illumina RNA PCR Primer (100% over 36bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	43328	0.13493360620682132	Illumina RNA PCR Primer (100% over 35bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	42648	0.1328159259026153	Illumina RNA PCR Primer (100% over 33bp)
AGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGTA	40932	0.12747189737023656	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	40862	0.12725390086833302	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	40282	0.12544764413827494	Illumina RNA PCR Primer (100% over 33bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT	39795	0.12393101133217443	Illumina RNA PCR Primer (100% over 44bp)
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA	38757	0.12069843463251878	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTTCAAATCGTTCGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	36830	0.11469730235868789	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	35981	0.11205331621417183	Illumina RNA PCR Primer (100% over 39bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	34886	0.10864322807725185	No Hit
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	34148	0.10634492210004003	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	33834	0.10536705207721549	Illumina RNA PCR Primer (100% over 37bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	33493	0.10430509768937098	Illumina RNA PCR Primer (100% over 38bp)
GTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTG	33436	0.10412758625210666	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	33274	0.10362308006198698	Illumina RNA PCR Primer (100% over 37bp)
AGCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	32863	0.10234312917223895	Illumina RNA PCR Primer (100% over 37bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	32777	0.1020753048984717	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	32594	0.10150539975778097	Illumina RNA PCR Primer (100% over 37bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	32487	0.10117217653344267	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGCATGATCGTCGGACTGTAGAACTCTGAA	32329	0.10068012728628893	Illumina DpnII expression Sequencing Primer (100% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	246295	44.366646	45
GCCGTAA	13050	44.136753	45
TGGAGCC	8705	44.098404	1
GGGGAAT	87190	43.792606	1
GCGTGAC	10650	43.613087	1
GTAACTG	46595	43.559772	17
TAGTAAC	47220	43.550232	15
TGACGCC	10540	43.444683	4
GTGACGC	10550	43.405197	3
TTAGTAA	49435	43.205517	14
TGGCGAC	9600	43.08292	1
TGGAGGT	6265	43.052956	1
CGTGGTG	8505	42.855934	10
CGTGACG	10865	42.83478	2
AATCGTT	36980	42.7903	6
GCTCACT	8340	42.65752	3
GTGAACC	5485	42.62542	3
TGGCGTC	6450	42.620956	1
AGTAACT	47755	42.529945	16
ACGTGGT	8635	42.52341	9

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content