H3NFYBCXY_l02n02_smallrna_drought_1h_rep3_shoot.3520000006c051.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l02n02_smallrna_drought_1h_rep3_shoot.3520000006c051.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32593768
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	4421921	13.566768346636081	Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	589086	1.8073577746518905	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	200962	0.6165657189435723	Illumina RNA PCR Primer (97% over 45bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	200217	0.6142800059201502	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	169245	0.5192557055692364	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	156882	0.4813251416651183	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	153824	0.4719429800199842	No Hit
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	151582	0.4650643644515111	Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	149145	0.45758747500442415	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	136155	0.4177332304752246	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	130400	0.4000764808781851	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	124484	0.38192577182239257	Illumina RNA PCR Primer (100% over 45bp)
GGCAATTATCCTAACCGTTGGACTACAGACGCGATCGTCGGACTGTAGAAC	118074	0.36225943560744495	No Hit
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	108243	0.3320972279117898	No Hit
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	107205	0.32891257003486063	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	104346	0.3201409545530299	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	93483	0.2868124974074799	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	91748	0.2814893939234028	Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCAGGTATCCTAACCGGACTAGACGACAATGGAGATCGTCGGACTGTAG	88994	0.27303992591467174	No Hit
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	87701	0.2690729098887861	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	85829	0.2633294806540931	Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	83634	0.2565950644307219	No Hit
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	82053	0.2517444439071911	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	77507	0.2377969923575574	No Hit
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	74429	0.22835346928897576	No Hit
GGCAATTATCCTAACCGTTGGACTACAAACGCGATCGTCGGACTGTAGAAC	72664	0.2229383236697273	No Hit
TCCTCTGAGCTACAGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	71007	0.21785452973709574	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT	68082	0.208880421557888	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	63294	0.1941904967845387	Illumina RNA PCR Primer (100% over 33bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	61452	0.18853910968501708	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	58545	0.17962022678691214	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGA	53393	0.163813524106817	No Hit
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	52828	0.162080063894423	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	52496	0.16106146426519327	Illumina RNA PCR Primer (100% over 34bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	52304	0.16047239460009657	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAAC	47737	0.14646051355584294	No Hit
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	46219	0.14180318151617205	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	45731	0.14030596278405125	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	45602	0.13991018160281438	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTG	42992	0.13190251584290594	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	42906	0.13163866172208133	Illumina RNA PCR Primer (100% over 37bp)
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA	42171	0.12938362941038298	Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGT	40196	0.12332418884493503	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	39738	0.12191901224798557	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	39386	0.12083905119530824	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC	39300	0.12057519707448369	No Hit
GAGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTC	38007	0.116608181048598	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	37905	0.11629523778901538	Illumina RNA PCR Primer (100% over 38bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC	37568	0.11526129780392375	No Hit
GCGTGACGCCCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	37114	0.11386839349166379	Illumina RNA PCR Primer (100% over 32bp)
AGCGAGAATCATACCACTAGACCAAACACCCGATCGTCGGACTGTAGAACT	36768	0.11280684086602077	No Hit
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	36444	0.11181278580617007	Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGG	36426	0.11175756052506725	No Hit
AGTCCCGCCTGACCTGGGGTCGCGATCGTCGGACTGTAGAACTCTGAACGT	35047	0.10752669037835699	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	33652	0.10324673109288868	Illumina RNA PCR Primer (100% over 38bp)
TGGCGGGACTGACGGGGCTCGAACCCGCAGCTTCCGCCTTGACAGGGCGGT	33040	0.10136907153539289	No Hit
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	33040	0.10136907153539289	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	22195	44.451366	45
GCCGTAT	461015	44.401897	45
TGGAGCC	7275	43.3279	1
GCTCACT	13010	43.311142	3
GGTGCAC	12935	43.253105	2
TAGTAAC	22860	43.24665	15
GTAACTG	22300	43.212746	17
GTGCACC	12960	43.13097	3
CGTGACG	29230	43.03164	2
TTAGTAA	23620	43.007732	14
GTGACGC	29080	42.98652	3
TGACGCC	29265	42.789772	4
ACGTCCC	15065	42.78828	9
TACGTCC	15070	42.77415	8
CGCCGTA	503715	42.687706	44
GCGTGAC	29425	42.681004	1
TGCTCAC	13230	42.66289	2
CTACGTC	15125	42.648487	7
TATGAGC	18860	42.523087	1
CTCACTC	13275	42.461613	4

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content