H3NFYBCXY_l02n02_smallrna_ctrl1h_rep2_shoot.3520000006bfb1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l02n02_smallrna_ctrl1h_rep2_shoot.3520000006bfb1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40082521
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	2463486	6.146035574957972	Illumina RNA PCR Primer (100% over 46bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	386453	0.9641434479632656	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	351275	0.8763795071672263	Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	317961	0.793265972467151	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	295276	0.7366702308969039	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	249900	0.6234637786380751	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	242427	0.6048197417522715	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	229913	0.573599150612308	Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	229878	0.573511830755356	Illumina DpnII expression Sequencing Primer (100% over 26bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	225429	0.5624122295102147	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	188832	0.47110809222803124	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	156684	0.3909035561909891	No Hit
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	133083	0.3320225292216525	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGA	121407	0.30289262494242813	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	113827	0.2839816387796566	Illumina RNA PCR Primer (97% over 45bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	112194	0.27990754373957666	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	101324	0.25278849102330664	No Hit
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	99825	0.2490487062927005	No Hit
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	96322	0.24030923603832205	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	96041	0.2396081823296494	Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	92879	0.23171945696729007	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	92785	0.23148494078004725	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	90889	0.22675469938629858	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTGATCGTCGGACTGTAGAACTCT	85177	0.212504098731714	Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGG	81225	0.20264443945529273	No Hit
TGGAGCCGACTATCGGATTTGAACCGATGGATCGTCGGACTGTAGAACTCT	80970	0.2020082519260702	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	76963	0.19201137573158136	No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	76532	0.19093609406454248	Illumina RNA PCR Primer (100% over 45bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	76121	0.18991070945861913	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	70781	0.1765881941407827	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	70381	0.17559025291847288	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTGATCGTCG	63570	0.158597808755592	No Hit
GTTCAAATCGTTCGTTCGTTAGGATCGTCGGACTGTAGAACTCTGAACGTG	61306	0.15294946143731827	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GAGATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGA	60598	0.15118310547382985	Illumina DpnII expression Sequencing Primer (100% over 24bp)
TGGTGCGCATCCGGGGAAACGAACCCCGGATCGTCGGACTGTAGAACTCTG	60502	0.15094359958047548	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	58432	0.14577925375502204	No Hit
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	54500	0.1359694915397163	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCCGAGATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACT	51327	0.12805332279374343	No Hit
ACGAATCCGTGCGACGCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGT	51163	0.1276441668925964	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCCGCTTCCGACTCACGGAATAAGTAAAATAACGTGATCGTCGGACTG	49520	0.12354512332195872	No Hit
CGTTGCCGAGAGTCGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	47575	0.11869263412847708	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTCAAATCGTTCGTTCGTTAGGGATCGTCGGACTGTAGAACTCTGAACGT	47233	0.11783939438340219	Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	47050	0.11738283627419543	No Hit
AGGGGGCACCCCTTCTCCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTA	46732	0.11658947300245909	No Hit
GTTCAAATCGTTCGTTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	44590	0.11124549775698989	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCAGGATCGAACTCTCCATGAGAGATCGTCGGACTGTAGAACTCTGAAC	43965	0.10968621459713077	Illumina DpnII expression Sequencing Primer (100% over 26bp)
CACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCT	43834	0.1093593888468243	No Hit
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	43088	0.10749822846721642	Illumina RNA PCR Primer (100% over 33bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	42032	0.10486366364031845	Illumina RNA PCR Primer (100% over 34bp)
GACTTCTCGCGACGTCGGGGGCGGCGAACCGCCCCCGTCGCCGCGATCCGA	42003	0.10479131290170096	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC	41440	0.10338671063129987	No Hit
GGGTGATGTCCTAGGCCTCTAGACGATAGGGTCGATCGTCGGACTGTAGAA	41116	0.1025783782412289	No Hit
GCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT	40797	0.10178252011643679	Illumina RNA PCR Primer (100% over 31bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGGAGCC	20610	44.372215	1
GCCGTAA	12790	44.29528	45
GCCGTAT	261015	44.09384	45
TGGTGCG	14065	43.667046	1
GGTGCAC	10100	43.42661	2
GTGCGCA	12250	43.42496	3
GTGCACC	10105	43.29004	3
TGACGCC	27595	43.234062	4
CGTGACG	27840	43.20599	2
TAGTAAC	21425	43.203022	15
GTGACGC	27565	43.19245	3
GGTGCGC	12330	43.165173	2
AATCGTT	72975	43.11198	6
GTAACTG	20735	43.08901	17
TTAGTAA	22500	43.008823	14
CTGGATG	37985	42.91379	22
GCTCACT	9675	42.91148	3
TGGCGTC	8850	42.865295	1
TTCAAAT	73640	42.84801	2
TCAAATC	73690	42.772503	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content