FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l02n02_smallrna_ctrl025h_rep1_shoot.3520000006bf98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l02n02_smallrna_ctrl025h_rep1_shoot.3520000006bf98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25496651
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT17706846.9447709034413965Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT12709984.984960573841639Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT3544541.3901982656467315Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC3498901.372297875513141Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA2439290.9567099616337847No Hit
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC2038470.7995050016568842Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG1908470.7485179131957369Illumina RNA PCR Primer (100% over 34bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG1486440.5829942136322139Illumina RNA PCR Primer (100% over 34bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA1485600.5826647585990804Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT1416190.5554415754445554Illumina RNA PCR Primer (100% over 38bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT1334040.523221657620838Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG1251500.4908487785317374Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT1186020.46516697428223025Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA1085690.4258167082414078Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTCCCGATCTGCACCAAGCGAGATCGTCGGACTGTAGAACTCTGAACGTGT1007160.3950165847271471Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT1006280.39467144135910237Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT1005590.39440081758188555Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT1000270.39231426903870625Illumina RNA PCR Primer (100% over 35bp)
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG976920.38315620353433866Illumina RNA PCR Primer (100% over 33bp)
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC903570.3543877193910683Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG900680.35325423719373966Illumina RNA PCR Primer (100% over 34bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA863610.33871507281485713Illumina RNA PCR Primer (100% over 45bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA838840.3290000714211447No Hit
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT836610.3281254467498496Illumina RNA PCR Primer (100% over 35bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG763670.2995177680394182Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA754300.2958427755864878Illumina RNA PCR Primer (97% over 45bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT749420.29392879872733085No Hit
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT716900.2811741824445885Illumina RNA PCR Primer (100% over 38bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA687970.2698275942201193No Hit
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT672240.2636581565163205Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG669130.26243838847698076Illumina RNA PCR Primer (100% over 34bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT658590.2583045122279001Illumina RNA PCR Primer (100% over 35bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG643650.2524449191385959Illumina RNA PCR Primer (100% over 36bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA635530.24926018715163809Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTTCAAATCGTTCGTTCGTTAGGATCGTCGGACTGTAGAACTCTGAACGTG626980.24590680556438568Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC619320.2429024894289058Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCT609600.23909022404550306Illumina DpnII expression Sequencing Primer (100% over 22bp)
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC595700.2336385276638881No Hit
CTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAA579370.22723376493642244Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT566520.22219388734622442Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC552680.2167657234669761Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA511200.20049692016414233Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG509260.1997360359209529Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGGCGGTGCTCTGACCAATTGAACTACAATCCCGATCGTCGGACTGTAGAA505350.19820250118339072No Hit
GGCCGGCCGCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG498310.19544135423903317Illumina RNA PCR Primer (100% over 33bp)
GGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAAC494550.1939666507573877No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG485800.1905348274955797Illumina RNA PCR Primer (100% over 34bp)
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT484720.19011124245297942Illumina RNA PCR Primer (100% over 35bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG465870.18271811462611307Illumina RNA PCR Primer (100% over 37bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT465090.18241219209534618Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG458260.17973340890927203Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG456480.1790352780057271Illumina RNA PCR Primer (100% over 37bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG450850.17682714486698664No Hit
GGCTGCGCCGACGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT442630.17360319204275104Illumina RNA PCR Primer (100% over 31bp)
TCAGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAG438770.17208926772382774No Hit
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG438370.1719323843747165Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCCAGGAGCGCATCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTG425120.16673562343540727Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACGGGAACGTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC424720.16657874008629603Illumina RNA PCR Primer (100% over 32bp)
AGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGTA422500.16570803749872876Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCACCTGACTTCGCAACCATGATCGTCGGACTGTAGAACTCTGAACGTGT421960.1654962449774286Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG412030.1616016158357425Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA411580.1614251220679924Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTCAAATCGTTCGTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT410660.16106429036503658Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG409030.16042499071740834Illumina RNA PCR Primer (100% over 36bp)
ACTGAGCTATAGCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC409010.1604171465499528Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC403140.1581148834017456Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACGGGAACGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG402530.15787563629435097Illumina RNA PCR Primer (100% over 33bp)
TTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTG398630.15634602364051653Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT395300.15503996975916562Illumina RNA PCR Primer (100% over 35bp)
GTGCTCACTCTCTTCTGTCAGATCGTCGGACTGTAGAACTCTGAACGTGTA394310.15465168347011535Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGGACCAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT380380.14918822083731703Illumina RNA PCR Primer (100% over 35bp)
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC379580.14887445413909456Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC379380.14879601246453897Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG371540.14572109882195902Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCAATTATCCTAACCGTTGGACTACAGACGCGATCGTCGGACTGTAGAAC364610.1430030947986071No Hit
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT355100.13927319317348777Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC349510.13708074836965842Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCAGATCATGAATTAGTAACTGCATGATCGTCGGACTGTAGAACTCTGAA337440.1323467933102273Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG332250.13031123185550916Illumina RNA PCR Primer (100% over 37bp)
AAGGGTCGTCATCAGCTCTCGGCCTTGGATCGTCGGACTGTAGAACTCTGA332150.13027201101823138Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT330400.12958564636586978Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT323930.12704805819399576Illumina RNA PCR Primer (100% over 38bp)
AGGAGATGTTTTACCATTGAACTACGCTCGCGATCGTCGGACTGTAGAACT311780.12228272646474236No Hit
AGCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAAC305660.11988241122334066No Hit
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA303850.11917251406861239Illumina DpnII expression Sequencing Primer (100% over 24bp)
GATCGTCGGACTGTAGAACTGATCGTCGGACTGTAGAACTCTGAACGTGTA303610.11907838405914564Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCTCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG303190.11891365654257886Illumina RNA PCR Primer (100% over 37bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT301250.11815277229938943Illumina RNA PCR Primer (100% over 38bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG301190.11812923979702275Illumina RNA PCR Primer (100% over 37bp)
CAGTTCAAGAAAGCCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT297130.11653687380354386Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG295900.11605445750502684Illumina RNA PCR Primer (100% over 36bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC289800.11366198643108069Illumina RNA PCR Primer (100% over 39bp)
CAGAGCTCCCTTCAATCCAAAGATCGTCGGACTGTAGAACTCTGAACGTGT286840.11250104964765766Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTGAGCTACAGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGA285990.1121676725307963Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTG285770.11208138668878512Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG283340.11112832034293446Illumina RNA PCR Primer (100% over 37bp)
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG283000.11099496949618991Illumina RNA PCR Primer (100% over 36bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT278700.10930847349324427Illumina RNA PCR Primer (100% over 35bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC276780.10855543341751041Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG273340.1072062366151539Illumina RNA PCR Primer (100% over 37bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG271110.10633161194385883Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC270690.10616688442729204Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGGGTCACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC268560.10533148059327477Illumina RNA PCR Primer (100% over 32bp)
TATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC262840.10308804870098429Illumina DpnII expression Sequencing Primer (100% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT1368750.044.4979551
TAGTAAC530800.044.15085215
GTAACTG525650.044.14254417
GCCGTAT1877750.044.09053845
TTAGTAA552150.043.98396314
ATTAGTA564500.043.9144913
AGTAACT530300.043.7469916
TAACTGG417850.043.70631418
GCCGTAA81350.043.17337845
ACGGGAA306400.043.169173
AACTGGA423350.043.138519
TGCTCAC160250.043.0859222
CGTGGTG141850.043.07946810
AATCGTT357450.043.079236
ACGTGGT142500.043.072439
GCTCACT160850.042.9487153
GGCCGCC517400.042.8507421
TTCGCAA48950.042.838311
CGTTAGG231550.042.79288517
CTCACTC161750.042.792134
Produced by FastQC (version 0.11.2)