FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l02n01_smallrna_heat_1h_rep1_shoot.3510000006bfeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l02n01_smallrna_heat_1h_rep1_shoot.3510000006bfeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32110607
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCC24895077.752911678063263RNA PCR Primer, Index 10 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7960352.4790406484685885RNA PCR Primer, Index 1 (100% over 30bp)
TAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA3924211.2220915039071045RNA PCR Primer, Index 7 (97% over 35bp)
CAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA3222091.0034347840263498RNA PCR Primer, Index 1 (100% over 28bp)
AGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA1788020.5568315790480074RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT1725650.5374080907284001RNA PCR Primer, Index 10 (100% over 43bp)
ATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAAC1570260.489015981541551RNA PCR Primer, Index 1 (100% over 24bp)
GGTAGTTCGACCGTGGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1518010.47274409979232096RNA PCR Primer, Index 1 (100% over 31bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT1464550.4560953955183719RNA PCR Primer, Index 10 (100% over 39bp)
TCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACT1425240.4438533348186162RNA PCR Primer, Index 1 (100% over 25bp)
CAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCC1391340.4332960756550009RNA PCR Primer, Index 1 (100% over 27bp)
CACACGACTCTCGGCAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1359050.42324020844576377RNA PCR Primer, Index 1 (100% over 32bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT1341060.4176376983468422RNA PCR Primer, Index 10 (100% over 39bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1265390.3940722764910673RNA PCR Primer, Index 1 (100% over 30bp)
CCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTC1184700.3689435082930696RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1160220.361319859197928RNA PCR Primer, Index 7 (97% over 35bp)
GATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAA1106450.344574613615993RNA PCR Primer, Index 1 (100% over 23bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA1066360.3320896425284019No Hit
GCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1027340.3199378946651491RNA PCR Primer, Index 1 (100% over 29bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA1013760.31570876252822005RNA PCR Primer, Index 10 (100% over 40bp)
TATCGAGTAGACCCTGTCGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1004290.31275958128103903RNA PCR Primer, Index 1 (100% over 28bp)
TATCGAGTAGACCCTGTCGTTGTGAGATGGAATTCTCGGGTGCCAAGGAAC962420.2997202762314646RNA PCR Primer, Index 1 (100% over 24bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT921560.2869955089917796RNA PCR Primer, Index 10 (100% over 43bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA892280.2778770267407278RNA PCR Primer, Index 10 (100% over 40bp)
TTCGGACCAGGCTTCATTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT871120.27128730391175726RNA PCR Primer, Index 1 (100% over 30bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTTGGAATTCTCGGGTGCCAAGG858960.26750039325011826Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT831460.2589362449610498RNA PCR Primer, Index 10 (100% over 39bp)
TATCGAGTAGACCCTGTCGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA806930.2512970246872007RNA PCR Primer, Index 1 (100% over 28bp)
AGGGCTATAGCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA784250.24423393802552534RNA PCR Primer, Index 7 (97% over 35bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGATGGAATTCTCGGGTGC781940.2435145495692436No Hit
CGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA769340.23959061253497949RNA PCR Primer, Index 7 (97% over 35bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTTGGAATTCTCGGGTGCCA761210.2370587388771567No Hit
TATCGAGTAGACCCTGTCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG680350.21187702867155392RNA PCR Primer, Index 1 (100% over 29bp)
TCATGTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA677190.21089293017724642RNA PCR Primer, Index 10 (100% over 40bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGGAATTCTCGGGTGCCAA634590.19762628591854398No Hit
CACACGACTCTCGGCAACGGATATCTGGAATTCTCGGGTGCCAAGGAACTC622630.19390165997173456RNA PCR Primer, Index 1 (100% over 26bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT573230.1785173354088261RNA PCR Primer, Index 10 (100% over 41bp)
AGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG558420.17390515227569509RNA PCR Primer, Index 1 (100% over 29bp)
TATCGAGTAGACCCTGTCGTTGTGAGTGGAATTCTCGGGTGCCAAGGAACT556790.17339753184983392RNA PCR Primer, Index 1 (100% over 25bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT554320.1726283156216885RNA PCR Primer, Index 10 (100% over 39bp)
GCGACCCCAGGTCAGGCGGGACTTGGAATTCTCGGGTGCCAAGGAACTCCA553950.17251308889925376RNA PCR Primer, Index 1 (100% over 28bp)
AGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGA545880.16999990065587983RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA543580.1692836264353396RNA PCR Primer, Index 10 (100% over 40bp)
TAATTCATGATCTGGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA530220.16512300748472306RNA PCR Primer, Index 1 (100% over 32bp)
CACACGACTCTCGGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC524460.16332920769763087RNA PCR Primer, Index 1 (100% over 33bp)
TTGACAGAAGAGAGTGAGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGT523840.16313612508165914RNA PCR Primer, Index 1 (100% over 30bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT520360.1620523710436243No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA503990.15695436713482247RNA PCR Primer, Index 7 (97% over 35bp)
CTGGGCGTCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT500730.15593912628310017RNA PCR Primer, Index 10 (100% over 38bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT497900.1550577975682615RNA PCR Primer, Index 10 (100% over 39bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTGGAATTCTCGGGTGCCAAGGA462740.14410814470121974RNA PCR Primer, Index 1 (100% over 22bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT453830.14133336065556157RNA PCR Primer, Index 10 (100% over 38bp)
TCTGTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA448670.13972641501295818RNA PCR Primer, Index 10 (100% over 40bp)
GGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT448510.1396765872410945RNA PCR Primer, Index 10 (100% over 41bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT446930.13918453799394076RNA PCR Primer, Index 3 (100% over 34bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT446340.13900079808519347RNA PCR Primer, Index 10 (100% over 38bp)
GCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC443180.13801669959088597RNA PCR Primer, Index 10 (100% over 42bp)
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTA441420.1374685941003856RNA PCR Primer, Index 10 (100% over 40bp)
TCCACAGGCTTTCTTGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC420010.1308010153778781RNA PCR Primer, Index 1 (100% over 31bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG411030.12800443168202957RNA PCR Primer, Index 10 (100% over 49bp)
TCCGTTGTAGTCTAGCTGGTTAGGATATGGAATTCTCGGGTGCCAAGGAAC393510.12254829066295757RNA PCR Primer, Index 1 (100% over 24bp)
AACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA383480.11942471221425369RNA PCR Primer, Index 7 (97% over 35bp)
GAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT364460.1135014358339598RNA PCR Primer, Index 3 (100% over 34bp)
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGTTTACACCGAGATGGAATT354250.11032180114190926No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTGGAATTCTCGGGTGCCAAGGAA353260.11001349180350282RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTC353100.10996366403163915RNA PCR Primer, Index 10 (100% over 44bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA343660.10702382549168256RNA PCR Primer, Index 1 (100% over 32bp)
GGGGACGTAGCTCATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA342610.10669683073882721RNA PCR Primer, Index 7 (97% over 35bp)
CTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG337330.10505251426732606RNA PCR Primer, Index 1 (100% over 29bp)
TCCACAGGCTTTCTTGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT332670.10360128041179664RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC330760.10300646138517407RNA PCR Primer, Index 10 (100% over 42bp)
GCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC327770.1020753048984717RNA PCR Primer, Index 10 (100% over 42bp)
ATGCAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACT327390.10195696394029549RNA PCR Primer, Index 1 (100% over 25bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT327190.1018946792254659RNA PCR Primer, Index 10 (100% over 43bp)
GTCGGCGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC325180.10126871784142853RNA PCR Primer, Index 10 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC324470.10104760710378348RNA PCR Primer, Index 10 (100% over 37bp)
GCCGTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG322890.10055555785662976RNA PCR Primer, Index 7 (97% over 36bp)
GGCGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC322420.1004091887767802RNA PCR Primer, Index 10 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG841100.044.8105471
CACACGA499900.044.301851
AGTATTC138300.044.2798921
AGTAGAC850750.044.2313966
TGTCGTT806450.044.2237915
TAGACCC848200.044.1893168
GTAGACC852650.044.138117
TCGAGTA853800.044.105013
AGTATAG147900.044.0982449
TAGTTCG198250.044.06513
GAGTAGA867800.044.0571635
CGACCCC108750.043.981962
TTCCCGC137550.043.9488625
TCCGTTG421500.043.9405021
AGCTCAG590950.043.9373559
GTCAGGA283800.043.93681
TAGTATA158750.043.8902478
TTCGACC199150.043.865966
GTATGCC2762150.043.8286145
TTGTGAG457900.043.82629420
Produced by FastQC (version 0.11.2)