FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l02n01_smallrna_ctrl025h_rep1_shoot.3510000006bf9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l02n01_smallrna_ctrl025h_rep1_shoot.3510000006bf9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25496651
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC18609047.29862129736176RNA PCR Primer, Index 9 (100% over 51bp)
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT12812155.025032503288373RNA PCR Primer, Index 1 (100% over 30bp)
CAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA3814241.495976863784973RNA PCR Primer, Index 1 (100% over 28bp)
TAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA3590171.408094733696594RNA PCR Primer, Index 7 (97% over 35bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA2481300.9731866353741909No Hit
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA2065640.8101613031452641RNA PCR Primer, Index 7 (97% over 35bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1924350.7547461821554525RNA PCR Primer, Index 9 (100% over 39bp)
GGTAGTTCGACCGTGGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1501470.5888891054750681RNA PCR Primer, Index 1 (100% over 31bp)
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1491660.5850415413381154RNA PCR Primer, Index 9 (100% over 39bp)
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT1372400.5382667708006044RNA PCR Primer, Index 9 (100% over 43bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1339240.525261141159284RNA PCR Primer, Index 1 (100% over 30bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1291330.5064704380194873RNA PCR Primer, Index 6 (100% over 34bp)
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1282120.5028581989062014RNA PCR Primer, Index 9 (100% over 39bp)
TATCGAGTAGACCCTGTCGTTGTGAGATGGAATTCTCGGGTGCCAAGGAAC1102640.43246464015999586RNA PCR Primer, Index 1 (100% over 24bp)
TTGACAGAAGAGAGTGAGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1075820.4219456116020885RNA PCR Primer, Index 1 (100% over 30bp)
GCCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA1060710.416019343089412RNA PCR Primer, Index 9 (100% over 38bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA1041110.40833205898296215RNA PCR Primer, Index 9 (100% over 40bp)
TCGCTTGGTGCAGATCGGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT1023660.401488022877985RNA PCR Primer, Index 1 (100% over 30bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG1019670.39992311147060056RNA PCR Primer, Index 9 (100% over 39bp)
TTCGGACCAGGCTTCATTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1011380.3966717040602705RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA931180.3652165925634704RNA PCR Primer, Index 7 (97% over 35bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTTGGAATTCTCGGGTGCCAAGG916710.3595413374093719Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA866950.34002504877993583RNA PCR Primer, Index 9 (100% over 40bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTTGGAATTCTCGGGTGCCA855550.335553873330266No Hit
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG819630.3214657485800782No Hit
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA808090.3169396639582195RNA PCR Primer, Index 1 (100% over 32bp)
GCCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG742430.2911872622016123RNA PCR Primer, Index 9 (100% over 39bp)
TCCACAGGCTTTCTTGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT721090.28281753552652855RNA PCR Primer, Index 1 (100% over 30bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT695400.2727417024298603RNA PCR Primer, Index 9 (100% over 43bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC694360.27233380572217114No Hit
TCATGTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA684090.26830582573374046RNA PCR Primer, Index 9 (100% over 40bp)
CTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG682350.26762338316510664RNA PCR Primer, Index 1 (100% over 29bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTGGAATTCTCGGGTGCCAAGGA644570.2528057508415517RNA PCR Primer, Index 1 (100% over 22bp)
GGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT643290.25230372412439583RNA PCR Primer, Index 9 (100% over 41bp)
ATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAAC640850.25134673569481736RNA PCR Primer, Index 1 (100% over 24bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGGAATTCTCGGGTGCCAA607840.23839993730941372No Hit
GCCGCGGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA602620.23635260960351226RNA PCR Primer, Index 9 (100% over 38bp)
TATCGAGTAGACCCTGTCGTTGTGAGTGGAATTCTCGGGTGCCAAGGAACT589570.23123429033875859RNA PCR Primer, Index 1 (100% over 25bp)
AGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA566520.22219388734622442RNA PCR Primer, Index 1 (100% over 28bp)
AGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG563220.22089959971605683RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATGTAGCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA560220.21972297459772264RNA PCR Primer, Index 7 (97% over 35bp)
GCCGTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT539370.21154543002530019RNA PCR Primer, Index 9 (100% over 36bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTGGAATTCTCGGGTGCCAA516270.20248541661412708No Hit
TCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACT512340.20094403770910932RNA PCR Primer, Index 1 (100% over 25bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGGAATTCTCGGGTGCCA510070.20005372470290314No Hit
TCTGTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA504470.19785735781534605RNA PCR Primer, Index 9 (100% over 40bp)
GCTGGTCCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA503070.19730826609345675RNA PCR Primer, Index 9 (100% over 40bp)
CCCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG493530.1935665982171541RNA PCR Primer, Index 9 (100% over 39bp)
GAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG489830.19211542723787528RNA PCR Primer, Index 6 (100% over 34bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGATGGAATTCTCGGGTGC477420.1872481213316996No Hit
CACACGACTCTCGGCAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA465230.1824671012675351RNA PCR Primer, Index 1 (100% over 32bp)
GCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC462910.18155717784269RNA PCR Primer, Index 9 (100% over 42bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT457300.1793568888714051No Hit
GCCGGCGATGCGCTCCTGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG455210.17853717337229896RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT449980.17648592358266976RNA PCR Primer, Index 9 (100% over 43bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA447740.17560737682764688RNA PCR Primer, Index 9 (100% over 38bp)
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC439310.1723010602451279RNA PCR Primer, Index 9 (100% over 42bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC439020.17218731981702226RNA PCR Primer, Index 9 (100% over 37bp)
TCCACAGGCTTTCTTGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC436570.171226409303716RNA PCR Primer, Index 1 (100% over 31bp)
ACCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC435470.17079498009366015RNA PCR Primer, Index 1 (100% over 33bp)
ATGGTTGCGAAGTCAGGTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT432840.16976347207325385RNA PCR Primer, Index 1 (100% over 30bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT432000.1694340170401203RNA PCR Primer, Index 9 (100% over 41bp)
TGACAGAAGAGAGTGAGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC430420.16881432781113095RNA PCR Primer, Index 1 (100% over 31bp)
CAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCC423790.16621398629961245RNA PCR Primer, Index 1 (100% over 27bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATGGAATTCTCGGGTGCCAAGGAA419880.16468045156205025RNA PCR Primer, Index 1 (100% over 23bp)
AGGGCTATAGCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA416940.16352735894608275RNA PCR Primer, Index 7 (97% over 35bp)
GGGCCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT415600.16300179972656015RNA PCR Primer, Index 9 (100% over 41bp)
CGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA403390.15821293549494012RNA PCR Primer, Index 7 (97% over 35bp)
CCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA401660.15753441501003407RNA PCR Primer, Index 9 (100% over 40bp)
GATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAA388530.1523847190754582RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA385530.151208093957124RNA PCR Primer, Index 7 (97% over 35bp)
GGGGACGTAGCTCATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA381600.14966671505210624RNA PCR Primer, Index 7 (97% over 35bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCAA376580.14769782902076042No Hit
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA365480.14334431608292397RNA PCR Primer, Index 9 (100% over 40bp)
TCCGTTGTAGTCTAGCTGGTTAGGATACTTGGAATTCTCGGGTGCCAAGGA353290.1385632960187595RNA PCR Primer, Index 1 (100% over 22bp)
CCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTC352900.13841033475337605RNA PCR Primer, Index 1 (100% over 26bp)
GCGAGCGTAGTTCAATGGTAAAACATCTCCTTGGAATTCTCGGGTGCCAAG348860.1368258129273527No Hit
CAAGGCCGAGAGCTGATGACGACCCTTTGGAATTCTCGGGTGCCAAGGAAC348360.13662970874096367RNA PCR Primer, Index 1 (100% over 24bp)
ATGCAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACT344200.13499812191020694RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGCATCCTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAG330500.1296248672031476No Hit
GGCGCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC322090.12632639478808413RNA PCR Primer, Index 9 (100% over 42bp)
GGCCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT318450.124898756311172RNA PCR Primer, Index 9 (100% over 43bp)
CAGGCTTTCTTGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG312510.12256903857687035RNA PCR Primer, Index 6 (100% over 34bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC311370.12212192103190336RNA PCR Primer, Index 1 (100% over 31bp)
TCCGTTGTAGTCTAGCTGGTTAGGATATGGAATTCTCGGGTGCCAAGGAAC310100.12162381639847523RNA PCR Primer, Index 1 (100% over 24bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTTGGAATTCTCGGGTGCCAA309710.12147085513309179No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTGGAATTCTCGGGTGCCAAGGAA302040.1184626169138841RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGATTGAAGGGAGCTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT295190.11577598956035443RNA PCR Primer, Index 1 (100% over 30bp)
GCGCCTGTAGCTCAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC293600.1151523782476373RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC292170.11459152027456468RNA PCR Primer, Index 9 (100% over 42bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT290820.1140620389713143RNA PCR Primer, Index 9 (100% over 43bp)
GCCGAGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC289730.11363453184498623RNA PCR Primer, Index 9 (100% over 42bp)
CACACGACTCTCGGCAACGGATATCTGGAATTCTCGGGTGCCAAGGAACTC289360.11348941474705834RNA PCR Primer, Index 1 (100% over 26bp)
CGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA288460.11313642721155812RNA PCR Primer, Index 7 (97% over 35bp)
GCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG285820.11210099710742404RNA PCR Primer, Index 1 (100% over 29bp)
CCCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA285610.11201863334914064RNA PCR Primer, Index 9 (100% over 40bp)
TCGTGACCCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC285060.1118029187441127RNA PCR Primer, Index 9 (100% over 37bp)
GGCGGACTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT283200.11107341117074553RNA PCR Primer, Index 9 (100% over 41bp)
TCCGTTGTAGTCTAGCTGGTTAGGATACTCGTGGAATTCTCGGGTGCCAAG280330.1099477731408725No Hit
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC280120.10986540938258912RNA PCR Primer, Index 9 (100% over 44bp)
GTCGGCGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC272890.10702974284740377RNA PCR Primer, Index 9 (100% over 42bp)
GCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATC269500.10570015646368615RNA PCR Primer, Index 9 (100% over 42bp)
GAGGCATCCTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGG267330.10484906429475778Illumina Small RNA Adapter 2 (100% over 21bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATATGGAATTCTCGGGTGCCAAGG266840.10465688219209653Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGAT266570.10455098593144645RNA PCR Primer, Index 9 (100% over 41bp)
CCGACCTGCTCGACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA266070.10435488174505743RNA PCR Primer, Index 7 (97% over 35bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA261030.10237815154625601RNA PCR Primer, Index 9 (100% over 40bp)
GGCGGACTGCTCGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG260550.10218989152732255RNA PCR Primer, Index 6 (100% over 34bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTTGGAATTCTCGGGTG256180.10047594093828244No Hit
GCCGGCGGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA255580.10024061591461561RNA PCR Primer, Index 9 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTC336700.044.51445421
TATCGAG584600.044.407871
TCCCCTG1360850.044.3871617
AGTATAG349600.044.3654069
TTCCCGC336050.044.26581625
TCGAGTA586000.044.2622533
TTCGACC214600.044.2407266
ATTCCCC1368900.044.1748415
TTCCCCT1368750.044.14974616
TATTCCC339000.044.13943523
TAGTATA358500.044.1234178
TGAGTAT340550.044.07726719
TCCGTTG466750.044.0723761
TCGACCG215200.044.0546537
TCCCGCC333450.044.04421626
TATAGTG352200.044.0258111
CGAGTAG591100.044.005964
TAGCTCA721300.044.0038538
AGCTCAG611300.043.983979
CGACCGT215500.043.9830558
Produced by FastQC (version 0.11.2)