H3NFYBCXY_l01n02_smallrna_salt24h_rep2_shoot.3520000006bfa5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_salt24h_rep2_shoot.3520000006bfa5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26630069
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	6188455	23.238599194016356	Illumina RNA PCR Primer (100% over 46bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	398391	1.4960194057326701	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	341964	1.284127352430067	Illumina RNA PCR Primer (97% over 45bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	262672	0.986373711611487	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	218591	0.8208427848985296	Illumina RNA PCR Primer (100% over 38bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	210790	0.7915488315107256	Illumina RNA PCR Primer (100% over 38bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	201109	0.7551951893177595	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	186585	0.7006553381442608	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	149994	0.5632505120433597	Illumina RNA PCR Primer (100% over 45bp)
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	146055	0.5484589619350968	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	141450	0.5311664795160689	Illumina RNA PCR Primer (100% over 37bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	135298	0.5080647744472611	Illumina RNA PCR Primer (100% over 39bp)
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	131613	0.494227033358419	Illumina RNA PCR Primer (100% over 35bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	124062	0.46587186837555694	Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	107904	0.4051960961873587	Illumina RNA PCR Primer (100% over 38bp)
GGCACGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	104892	0.39388557348461994	Illumina RNA PCR Primer (100% over 35bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	104573	0.39268767947991423	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	101146	0.3798187680249721	Illumina RNA PCR Primer (100% over 36bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	98969	0.37164379859473895	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	83542	0.31371304370259046	No Hit
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	81794	0.30714903517523745	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	78116	0.2933375801617337	Illumina RNA PCR Primer (100% over 34bp)
GCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	75584	0.2838295311964832	Illumina RNA PCR Primer (100% over 39bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	65754	0.24691637111417175	No Hit
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	64656	0.24279321243966734	Illumina RNA PCR Primer (100% over 34bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	62083	0.23313120217600636	No Hit
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	62064	0.2330598542572308	Illumina RNA PCR Primer (100% over 34bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT	60573	0.22746092021015793	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	59584	0.22374707328020818	Illumina RNA PCR Primer (100% over 34bp)
AGCCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	59491	0.22339784399356982	Illumina RNA PCR Primer (100% over 39bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	58653	0.2202510252602049	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGTTCAGTCATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	56218	0.2111072261960718	Illumina RNA PCR Primer (100% over 33bp)
GGCAATTATCCTAACCGTTGGACTACAGACGCGATCGTCGGACTGTAGAAC	53930	0.20251543471404448	No Hit
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	53588	0.20123117217608408	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	49009	0.18403632375117016	Illumina RNA PCR Primer (100% over 37bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	46988	0.17644715828562066	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCGAGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	46789	0.175699882715287	Illumina RNA PCR Primer (100% over 36bp)
GAGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTC	46538	0.17475733915672542	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCCAGGTATCCTAACCGGACTAGACGACAATGGAGATCGTCGGACTGTAG	45849	0.17217003831270583	No Hit
ACTGAGCTATAGCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	44757	0.16806941055992008	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	43875	0.1647573650672854	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCTCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	43812	0.16452079038924006	Illumina RNA PCR Primer (100% over 37bp)
AGCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	43390	0.16293611556169832	Illumina RNA PCR Primer (100% over 37bp)
TCCTCTGAGCTACAGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	42073	0.15799057824446494	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	40063	0.1504427194687329	No Hit
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG	39419	0.1480244005376028	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	38067	0.14294743284367756	Illumina RNA PCR Primer (100% over 38bp)
CATGCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAG	36395	0.13666881599142683	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	35756	0.1342692728283956	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCAATTATCCTAACCGTTGGACTACAAACGCGATCGTCGGACTGTAGAAC	35721	0.13413784245170374	No Hit
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA	35202	0.13218891772304459	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCCGGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	34991	0.1313965803092737	Illumina RNA PCR Primer (100% over 37bp)
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	34327	0.12890315830574828	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGCCGAGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	33851	0.1271157051827391	Illumina RNA PCR Primer (100% over 37bp)
AGGGTCGAGCAGGTCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	33515	0.12585397356649733	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	33312	0.12509167738168459	No Hit
GGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	33199	0.12466734502265091	Illumina RNA PCR Primer (100% over 39bp)
TCTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	32963	0.12378112876838585	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	30366	0.11402899481785045	Illumina RNA PCR Primer (100% over 38bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	30349	0.11396515720631441	Illumina RNA PCR Primer (97% over 46bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	28405	0.10666513856948699	Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG	28088	0.10547475487202079	Illumina RNA PCR Primer (100% over 40bp)
GGCGGACCAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	27780	0.1043181675571325	Illumina RNA PCR Primer (100% over 35bp)
AGCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	27572	0.10353709560422092	Illumina RNA PCR Primer (100% over 39bp)
AGGCCAGGAGCGCATCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTG	27536	0.10340191007390931	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	27491	0.10323292816101978	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	27131	0.10188107285790361	Illumina RNA PCR Primer (100% over 40bp)
AGGCTTATGTTGGATTGGCACGACAGATCGTCGGACTGTAGAACTCTGAAC	26836	0.10077330254007227	Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	26677	0.10017623311452928	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	36630	44.637947	45
GCCGTAT	646595	44.588478	45
GTAACTG	36080	43.91875	17
TAGTAAC	37255	43.80122	15
TTAGTAA	38960	43.564323	14
ATTAGTA	39935	43.52042	13
GGGCCCG	27030	43.328583	1
AGTAACT	36650	43.2664	16
CGCCGTA	707240	43.12321	44
TCGCCGT	709830	42.989223	43
TAACTGG	29830	42.908157	18
GGTCGCC	713210	42.877914	41
TGGAGCC	1835	42.844013	1
GTGACGC	3620	42.8439	3
GTCGCCG	716195	42.723713	42
GGCCGCC	57710	42.688232	1
AGGCCAG	6830	42.547077	1
TGGTCGC	720510	42.430775	40
TATGAGC	6670	42.250534	1
CAGTTCA	20165	42.193882	1

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content