H3NFYBCXY_l01n02_smallrna_salt24h_rep1_shoot.3520000006bf8b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_salt24h_rep1_shoot.3520000006bf8b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	36396323
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	1042410	2.864053052831738	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	419924	1.153753910800275	Illumina RNA PCR Primer (100% over 46bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	319578	0.8780502360087308	No Hit
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	289286	0.794822048370106	No Hit
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	280650	0.771094376758883	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	277078	0.7612801985519252	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	216873	0.5958651372557607	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	203325	0.5586415968448242	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	157938	0.43393943943183494	Illumina DpnII expression Sequencing Primer (100% over 24bp)
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	135878	0.37332892116601996	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGAGATGTTTTACCATTGAACTACGCTCGCGATCGTCGGACTGTAGAACT	134893	0.3706226038273152	No Hit
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	123237	0.3385973907309263	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCAATTATCCTAACCGTTGGACTACAGACGCGATCGTCGGACTGTAGAAC	122127	0.33554763210558386	No Hit
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	111778	0.30711344110227834	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	109644	0.3012502114568002	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAAC	103836	0.28529255551446775	No Hit
GGCAATTATCCTAACCGTTGGACTACAAACGCGATCGTCGGACTGTAGAAC	97480	0.26782925297151583	No Hit
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	96710	0.26571365464582786	No Hit
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	90442	0.24849213476866883	Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCCAGGTATCCTAACCGGACTAGACGACAATGGAGATCGTCGGACTGTAG	88702	0.24371143205867254	No Hit
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	85817	0.2357848071630752	Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	77316	0.21242805214142102	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	76494	0.2101695822404917	Illumina RNA PCR Primer (100% over 37bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	74688	0.20520754253115073	No Hit
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	72809	0.20004493311041338	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGGACCAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	71233	0.19571482536848572	Illumina RNA PCR Primer (100% over 35bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	70620	0.19403058929881462	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	66756	0.18341413224627112	No Hit
GTCCCGATCTGCACCAAGCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	66282	0.18211180288734113	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	65709	0.18053746802939408	No Hit
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	63307	0.1739379002653647	Illumina RNA PCR Primer (100% over 33bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	62950	0.17295703195072756	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT	61543	0.169091256828334	Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	61479	0.1689154148895755	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	60511	0.16625580556585345	Illumina RNA PCR Primer (100% over 34bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	57931	0.15916717740965206	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CTGCCTTGATCCACTTGGCTACATCCGCCGATCGTCGGACTGTAGAACTCT	57736	0.1586314090024973	Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	55060	0.1512790179381582	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATTCACAATCCACTGCCTTGATCCACTTGGCTACATCCGCCGATCGTCGG	54969	0.15102899268148598	No Hit
CCACGGGAACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	53790	0.14778965446591955	Illumina RNA PCR Primer (100% over 35bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	53475	0.14692418242359262	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	51707	0.14206654886538952	Illumina DpnII expression Sequencing Primer (100% over 26bp)
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA	51035	0.14022020850842543	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	51011	0.140154267781391	Illumina RNA PCR Primer (100% over 38bp)
ACTGAGCTATAGCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	50236	0.13802493180423747	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGAA	49588	0.13624453217430782	No Hit
TACGCCTAGGACACCAGAATAGATCGTCGGACTGTAGAACTCTGAACGTGT	48021	0.13193915220501806	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCGACGGGTTCGGGGCCGGGGATCGTCGGACTGTAGAACTCTGAACGTG	47896	0.1315957109183804	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTG	47631	0.13086761539070857	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	47583	0.1307357339366397	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	47060	0.1292987755933477	Illumina RNA PCR Primer (100% over 36bp)
GGCACGACCACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	46848	0.12871629917121022	Illumina RNA PCR Primer (100% over 35bp)
ACAACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTA	46328	0.12728758341879756	No Hit
AGATTTTGAGTCTCGCGTGTCTACCATTTCACCATCAAGGCGATCGTCGGA	45709	0.12558686216736786	No Hit
AGCGAGAATCATACCACTAGACCAAACACCCGATCGTCGGACTGTAGAACT	44913	0.12339982805405919	No Hit
TAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTGATCGTCG	44562	0.12243544492118064	No Hit
AGGCTGTACTCTCTCTCTTCTGATCGTCGGACTGTAGAACTCTGAACGTGT	44152	0.1213089575010091	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGT	44052	0.12103420447169898	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCTCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	41627	0.11437144351092829	Illumina RNA PCR Primer (100% over 37bp)
TGGTGCGCATCCGGGGAAACGAACCCCGGATCGTCGGACTGTAGAACTCTG	41580	0.11424230958715252	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGGCGGTGCTCTGACCAATTGAACTACAATCCCGATCGTCGGACTGTAGAA	40874	0.11230255320022299	No Hit
AGGCTTATGTTGGATTGGCACGACAGATCGTCGGACTGTAGAACTCTGAAC	40524	0.11134091759763755	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC	40373	0.11092604052337925	No Hit
AGTCCCGCCTGACCTGGGGTCGCGATCGTCGGACTGTAGAACTCTGAACGT	39525	0.10859613483482933	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCGTGACGCCCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	39234	0.10779660351953683	Illumina RNA PCR Primer (100% over 32bp)
GAGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTC	39156	0.10758229615667496	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG	39150	0.10756581097491635	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCTTGGCAAGGAGATGTTTTACCATTGAACTACGCTCGCGATCGTCGGAC	38865	0.10678276484138247	No Hit
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	38834	0.10669759140229632	Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	37578	0.10324669335416109	Illumina RNA PCR Primer (100% over 35bp)
CCACGGGAACGTGGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	37298	0.10247738487209272	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	36819	0.10116131786169719	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCAGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAG	36462	0.10018044954706001	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACGC	18450	43.5916	3
TAGTAAC	41600	43.380234	15
GTAACTG	41110	43.21896	17
TTAGTAA	42975	43.179966	14
TGACGCC	18730	43.011395	4
GCGTGAC	18990	42.915344	1
CGTGACG	19095	42.68814	2
GTGCACC	16615	42.62109	3
TGGCGAC	8250	42.59314	1
GGTGCAC	16690	42.500153	2
GCTGTAC	5525	42.4116	3
GCCGTAT	47820	42.359863	45
TGGCGCC	7615	42.329067	1
AGTAACT	42380	41.971657	16
CTGTACT	5605	41.926098	4
TGGAGCC	5775	41.657024	1
AATCGTT	34515	41.51245	6
CGTTAGG	27700	41.488792	17
GGGCTGG	69275	41.445213	19
GCCGTAA	2915	41.44426	45

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content