H3NFYBCXY_l01n02_smallrna_heat_1h_rep2_shoot.3520000006c02a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_heat_1h_rep2_shoot.3520000006c02a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32724954
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	721189	2.203789194019952	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	381759	1.166568484710475	No Hit
GTTCAAATCGTTCGTTCGTTAGGATGCCTCAGCTGCATACATCACTGCACT	325083	0.9933795476076146	No Hit
TGGCGGGACTGACGGGGCTCGAACCCGCAGCTTCCGCCTTGACAGGGCGGT	319265	0.975601065779955	No Hit
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	248396	0.7590415558720113	No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	246199	0.7523280246627696	Illumina RNA PCR Primer (100% over 46bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	210373	0.64285193494848	Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	209381	0.6398206090679303	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	205965	0.6293820917211984	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	193206	0.5903934960458614	Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	170885	0.5221856079614352	Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	157623	0.4816599589414243	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	151076	0.4616538192842074	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTTCAAATCGTTCGTTCGTTAGGATCGTCGGACTGTAGAACTCTGAACGTG	144779	0.4424116226412419	Illumina DpnII expression Sequencing Primer (96% over 29bp)
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	144421	0.4413176562448338	Illumina DpnII expression Sequencing Primer (100% over 21bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	144135	0.4404437054365302	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	142250	0.4346835751090743	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	141929	0.4337026722787754	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	125452	0.38335271609549093	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	122186	0.37337256455731	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	121001	0.3697514746697581	Illumina RNA PCR Primer (100% over 36bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	118224	0.3612655956674531	Illumina RNA PCR Primer (100% over 37bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	111346	0.3402479954593672	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	107533	0.3285963366060041	Illumina RNA PCR Primer (100% over 38bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	100265	0.306386985295686	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	93074	0.2844129284337573	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	92623	0.2830347752360477	Illumina RNA PCR Primer (100% over 34bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	91270	0.27890031564291884	Illumina RNA PCR Primer (100% over 34bp)
AAGTCCGTAGCGTCTACCGATTTCGCCATATCCCCGATCGTCGGACTGTAG	84076	0.25691709146481917	No Hit
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	83058	0.2538063155107873	Illumina RNA PCR Primer (100% over 34bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	77235	0.2360125548228425	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	73534	0.22470314243986408	Illumina RNA PCR Primer (100% over 35bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC	72775	0.22238381144859667	No Hit
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	72247	0.22077036380249762	Illumina RNA PCR Primer (100% over 35bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	71123	0.21733567601042314	Illumina RNA PCR Primer (100% over 38bp)
ATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCT	70076	0.21413628266673804	Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	69931	0.2136931957184722	No Hit
CTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAA	68819	0.2102951771910818	Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	67989	0.20775888638376694	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	66176	0.20221877164441543	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	65849	0.20121953418177457	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT	63089	0.19278560330443859	Illumina RNA PCR Primer (100% over 44bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	60079	0.18358772941285112	Illumina RNA PCR Primer (100% over 36bp)
CAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGTG	59075	0.1805197342676173	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACTTCTCGCGACGTCGGGGGCGGCGAACCGCCCCCGTCGCCGCGATCCGA	58269	0.17805678198967062	No Hit
AAGTCAGACAATTGTATGCCTCAAAATTTTAGCTCATAAGGTAACCTCGTA	57998	0.17722866776222207	No Hit
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	56926	0.17395288011711185	No Hit
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	56775	0.17349145853650397	No Hit
CAGTTCAAGAAAGCCTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	53234	0.16267096968264647	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	51734	0.15808731159713776	No Hit
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	51190	0.15642497159812663	Illumina RNA PCR Primer (100% over 35bp)
GTTCAAATCGTTCGTTCGTTAGGAGATCGTCGGACTGTAGAACTCTGAACG	50809	0.15526072244440742	Illumina DpnII expression Sequencing Primer (96% over 26bp)
GTTCAAATCGTTCGTTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	50272	0.1536197728497953	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTG	49889	0.15244941215196206	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	49500	0.15126071682178682	Illumina RNA PCR Primer (100% over 37bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	49115	0.1500842445798396	Illumina RNA PCR Primer (100% over 39bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	48630	0.14860219513219178	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	47361	0.14472442039185143	No Hit
GGGCGGTGCTCTGACCAATTGAACTACAATCCCGATCGTCGGACTGTAGAA	46857	0.1431843112751205	No Hit
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	46134	0.14097498807790532	No Hit
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT	44908	0.1372286115360162	No Hit
AGAATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAAC	44884	0.1371552730066481	No Hit
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	43420	0.1326816227151916	Illumina RNA PCR Primer (100% over 35bp)
AGCCGAGATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACT	43110	0.1317343333775198	No Hit
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	42715	0.13052730341500252	Illumina RNA PCR Primer (100% over 35bp)
CAATGATCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	42427	0.12964724106258482	Illumina RNA PCR Primer (100% over 36bp)
GTTCAAATCGTTCGTTCGTTAGGATGCCTCAGCTGCATACATCACGATCGT	42266	0.1291552617614069	No Hit
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	41741	0.1275509814314789	Illumina RNA PCR Primer (100% over 36bp)
CCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGGA	39136	0.11959069522297877	No Hit
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	38593	0.11793141099602462	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGGGACTGACGGGGCTCGAACCCGCAGCTTCCGCCTTGACAGGGCGGTG	38531	0.11774195312849027	No Hit
GTTCAAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	37912	0.11585043022520368	Illumina RNA PCR Primer (100% over 39bp)
ACGAATCCGTGCGACGCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGT	36566	0.11173736103647389	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG	36378	0.11116287588975679	Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	36271	0.11083590827965717	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTAAGAATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAG	35602	0.10879159677352031	No Hit
GCCAGATCATGAATTAGTAACTGCATGATCGTCGGACTGTAGAACTCTGAA	35422	0.10824155780325925	Illumina DpnII expression Sequencing Primer (100% over 25bp)
CACCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCT	35388	0.10813766155332105	No Hit
GTTCAAATCGTTCGTTCGTTAGATCGTCGGACTGTAGAACTCTGAACGTGT	35045	0.10708953173776807	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAGCTCGAGCAGTCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAG	34766	0.10623697133386345	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTGATCGTCGGACTGTAGAACTCT	33961	0.10377707482797378	Illumina DpnII expression Sequencing Primer (100% over 22bp)
CGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	33892	0.10356622655604039	No Hit
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	33720	0.10304063376223539	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATTCACAATCCACTGCCTTGATCCACTTGGCTACATCCGCCGATCGTCGG	33644	0.10280839508590295	No Hit
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	33535	0.10247531593168932	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	32805	0.10024460233007508	Illumina RNA PCR Primer (100% over 34bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGGCGGG	34090	44.30475	1
GTAACTG	26075	44.039818	17
TGGAGCC	8765	43.94634	1
TAGTAAC	26605	43.872456	15
TTAGTAA	27855	43.777004	14
AATCGTT	105800	43.76589	6
CTGCACT	33345	43.739315	45
CGTTAGG	82650	43.650105	17
GCGTGAC	51650	43.62094	1
ATCGTTC	106105	43.549026	7
CGTGACG	52095	43.52011	2
GTGACGC	51915	43.51918	3
TCAAATC	107530	43.512184	3
ATTAGTA	28650	43.504585	13
GCCGTAT	27220	43.440407	45
TTCAAAT	108070	43.415787	2
TGACGCC	52090	43.40688	4
GTTAGGA	81155	43.392868	18
CGGCCTC	11420	43.374813	1
TAGGATG	58450	43.373665	20

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content