H3NFYBCXY_l01n02_smallrna_drought_1h_rep2_shoot.3520000006c044.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_drought_1h_rep2_shoot.3520000006c044.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15062649
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	2041745	13.555019439143805	Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	429139	2.8490274187495177	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	113038	0.7504523274757315	Illumina RNA PCR Primer (97% over 45bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	102990	0.6837442736666041	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	92538	0.614354088713081	Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	87500	0.5809071166698501	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	81433	0.5406286769345817	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	72692	0.48259771571388277	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	68520	0.4549000643910643	No Hit
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	67894	0.4507440889049463	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	60651	0.4026582575216351	Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	60240	0.3999296538079059	Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	59826	0.39718113327874793	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	58744	0.3899978018474705	No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	55581	0.368998839447165	Illumina RNA PCR Primer (100% over 45bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	54114	0.3592595167025402	Illumina RNA PCR Primer (100% over 34bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	53238	0.35344380659736546	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	52727	0.35005130903601356	Illumina RNA PCR Primer (100% over 34bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	43161	0.2865432235724274	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	43027	0.28565360581661303	Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	42030	0.27903458415581484	Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	40961	0.2719375589247283	No Hit
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	38212	0.25368711705358066	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	34693	0.2303246925557384	Illumina RNA PCR Primer (100% over 38bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	31149	0.20679629459599036	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	31011	0.20588012108627107	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	30112	0.1999117153961431	Illumina RNA PCR Primer (100% over 36bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	28328	0.18806784915455443	Illumina RNA PCR Primer (100% over 39bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	28229	0.18741059424540796	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	27881	0.18510024365568103	Illumina RNA PCR Primer (100% over 38bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	26544	0.1762239829129657	Illumina RNA PCR Primer (100% over 35bp)
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	25917	0.17206136848837145	Illumina RNA PCR Primer (100% over 33bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT	25754	0.1709792215167465	Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	24617	0.16343074846927655	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	23727	0.15752209322543465	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT	23251	0.15436195851075066	No Hit
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	23160	0.15375781510941403	Illumina RNA PCR Primer (100% over 37bp)
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	21953	0.14574461636860822	Illumina RNA PCR Primer (100% over 35bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	21298	0.1413961116666796	Illumina RNA PCR Primer (100% over 35bp)
GCCCGGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	21002	0.13943098587771646	Illumina RNA PCR Primer (100% over 37bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	20956	0.13912559470781002	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC	20881	0.13862767432209303	No Hit
TATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGA	20824	0.1382492548289481	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	20662	0.13717374679579933	Illumina RNA PCR Primer (100% over 38bp)
GTCCCGATCTGCACCAAGCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	19991	0.13271901907825112	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTG	18256	0.12120046082199751	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	18104	0.12019134217361102	Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGT	17583	0.11673245522749684	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTCAGGGTCACGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	17094	0.11348601431262191	Illumina RNA PCR Primer (100% over 32bp)
GAGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTC	17012	0.1129416213575713	Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	16900	0.11219806024823388	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTCCCGCCTGACCTGGGGTCGCGATCGTCGGACTGTAGAACTCTGAACGT	16692	0.1108171610451787	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTGATCGTCG	16382	0.10875909011754839	No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	16254	0.10790930599259134	Illumina RNA PCR Primer (100% over 34bp)
ACGAATCCGTGCGACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	16140	0.10715246700630147	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCGGACCAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	15831	0.10510103501714739	Illumina RNA PCR Primer (100% over 35bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	15378	0.10209359588741662	Illumina RNA PCR Primer (100% over 35bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	12690	44.44448	45
GCCGTAT	215435	44.3205	45
GTGACGC	6055	43.494976	3
TGGAGCC	2610	43.239357	1
TGGCGAC	3335	43.160553	1
TGACGCC	6090	43.133408	4
CGTGACG	6170	43.126522	2
TAGTAAC	9995	43.106503	15
GTAACTG	9740	43.103485	17
TTAGTAA	10380	43.067688	14
GGTGCAC	5565	42.83935	2
GCGTGAC	6220	42.80644	1
GTGCACC	5555	42.790485	3
ATTAGTA	10650	42.73634	13
CGCCGTA	237785	42.573242	44
GGGGAAT	49390	42.406506	1
TGCTCAC	4825	42.271046	2
TCGCCGT	239925	42.211285	43
GGTCGCC	240750	42.178627	41
GGGCTGG	37435	42.143116	19

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content