H3NFYBCXY_l01n02_smallrna_ctrl1h_rep1_shoot.3520000006c01d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_ctrl1h_rep1_shoot.3520000006c01d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29254386
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	3727499	12.741675726846566	Illumina RNA PCR Primer (100% over 46bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	391515	1.3383121423228639	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	211113	0.7216456363158673	Illumina RNA PCR Primer (100% over 38bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	203882	0.6969279751760984	Illumina RNA PCR Primer (97% over 45bp)
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	188337	0.6437906439054984	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	171110	0.5849037474244033	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	158690	0.5424485750615309	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	156655	0.5354923531808188	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	139897	0.47820863510859535	Illumina RNA PCR Primer (100% over 34bp)
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	130031	0.44448377757783053	Illumina RNA PCR Primer (100% over 34bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	125851	0.4301953218228542	Illumina DpnII expression Sequencing Primer (96% over 28bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	123733	0.42295538180155273	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	123714	0.42289043427539375	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	123440	0.4219538225823642	Illumina RNA PCR Primer (100% over 38bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	120297	0.4112101344393282	No Hit
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	114518	0.3914558316144458	Illumina RNA PCR Primer (100% over 45bp)
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	91953	0.3143220985735267	Illumina RNA PCR Primer (100% over 39bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	89607	0.3063027882383175	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	85867	0.2935183804575492	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	82844	0.28318488721656987	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCGTCGGACTGTAGAACTCTGAACG	78905	0.26972023955655744	Illumina DpnII expression Sequencing Primer (96% over 26bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	78415	0.2680452770398258	Illumina RNA PCR Primer (100% over 35bp)
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	73348	0.25072479730048003	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGGATCGTCGGACTGTAGAACTCTGAAC	70872	0.2422611091547093	Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	69668	0.23814548697074006	No Hit
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	68008	0.23247112415895518	Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCCCAACAGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	66303	0.22664293825889903	Illumina RNA PCR Primer (100% over 35bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	65719	0.2246466564022229	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	61940	0.21172893527828612	Illumina RNA PCR Primer (100% over 36bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	60933	0.20828671639186	Illumina RNA PCR Primer (100% over 38bp)
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	59887	0.2047111841622654	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	57907	0.19794296827832925	No Hit
ACGAATCCGTGCGACGCGGGGCTGGATCGATCGTCGGACTGTAGAACTCTG	56744	0.19396749601922939	Illumina DpnII expression Sequencing Primer (100% over 23bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	56539	0.19326674639488245	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	55408	0.18940065944299772	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	50769	0.1735432081876543	Illumina RNA PCR Primer (100% over 37bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	48168	0.16465223368557455	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCTGACCTGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	46393	0.1585847674259853	Illumina RNA PCR Primer (100% over 35bp)
GCCCGGGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	46106	0.1576037179518996	Illumina RNA PCR Primer (100% over 37bp)
TCAGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAG	45655	0.15606206877833634	No Hit
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	44368	0.15166272845377782	Illumina RNA PCR Primer (100% over 33bp)
TGGTGCGCATCCGGGGAAACGAACCCCGGATCGTCGGACTGTAGAACTCTG	43248	0.1478342427012483	Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	41491	0.14182830567696755	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	39506	0.135042998338779	Illumina RNA PCR Primer (100% over 38bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT	39240	0.13413373297255324	No Hit
GTCCCGATCTGCACCAAGCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	38436	0.1313854271287731	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	38127	0.130329175255977	Illumina RNA PCR Primer (100% over 38bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC	35882	0.12265511229666554	No Hit
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	35658	0.12188941514615961	Illumina RNA PCR Primer (100% over 34bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	33858	0.11573649161530856	Illumina RNA PCR Primer (100% over 35bp)
TCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGT	33496	0.11449907032743739	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	33301	0.11383250361159519	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	32600	0.11143628172541374	Illumina RNA PCR Primer (100% over 35bp)
GCCCGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	31322	0.10706770601850948	Illumina RNA PCR Primer (100% over 39bp)
TAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTGATCGTCG	29858	0.10206332821341729	No Hit
AGTCCCGCCTGACCTGGGGTCGCGATCGTCGGACTGTAGAACTCTGAACGT	29325	0.10024137919011529	Illumina DpnII expression Sequencing Primer (96% over 28bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	389710	44.38551	45
GCCGTAA	21985	44.319736	45
TGGAGCC	4705	43.47061	1
GTAACTG	14250	43.308277	17
TAGTAAC	14675	43.157673	15
GTGCACC	7755	43.131638	3
GTGACGC	10115	42.90423	3
TTAGTAA	15415	42.89512	14
TGACGCC	10210	42.79126	4
GTGAACC	6700	42.73378	3
GGTGCAC	7895	42.570305	2
CGCCGTA	429810	42.564083	44
GCGTGAC	10355	42.548897	1
GCTCACT	8335	42.479763	3
CGTGACG	10385	42.312862	2
GGTGCGC	10540	42.309994	2
GTGCGCA	10485	42.29186	3
CTGTACT	3540	42.283764	4
TGGCGAC	6175	42.278156	1
GGTCGCC	434225	42.256416	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content