H3NFYBCXY_l01n02_smallrna_ctrl025h_rep3_shoot.3520000006bff5.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_ctrl025h_rep3_shoot.3520000006bff5.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743609
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	245507	33.01560363040254	Illumina RNA PCR Primer (100% over 46bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	13666	1.8377937867884868	Illumina RNA PCR Primer (97% over 45bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	13023	1.7513236122747304	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	8114	1.091164846041401	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	7275	0.9783367334176967	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	5749	0.7731213581331049	Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAA	4560	0.6132254988844944	Illumina DpnII expression Sequencing Primer (100% over 25bp)
TATGAGCTACGTCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	3255	0.4377300436116292	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	2707	0.36403540032463294	Illumina RNA PCR Primer (100% over 45bp)
TGGTGCCTTGAAGAGGACTCGAACCTCCACGCTGATCGTCGGACTGTAGAA	2547	0.3425187161532472	No Hit
TCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGT	2490	0.334853397417191	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGTG	2295	0.3086299385833146	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	2059	0.2768928294305206	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAAC	2039	0.2742032439090974	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCGTGACGCCCAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	1864	0.2506693705966442	Illumina RNA PCR Primer (100% over 33bp)
TTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTG	1840	0.24744186797093634	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTGTAGCAATGAAATACCACAACCTACCCGATATGATCGTCGGACTGTAGA	1692	0.22753893511240447	No Hit
TCAGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAG	1680	0.22592518379955057	No Hit
TACGCCTAGGACACCAGAATAGATCGTCGGACTGTAGAACTCTGAACGTGT	1642	0.22081497130884645	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCGTGACGCCCAGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC	1585	0.21314965257279028	Illumina RNA PCR Primer (100% over 32bp)
CGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG	1581	0.21261173546850565	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TATGAGCTACACCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	1487	0.1999706835178165	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CATGCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAG	1210	0.16271992404610489	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTGCGCTACATCCCTGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	1181	0.15882002504004122	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACGT	1175	0.15801314938361424	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	1167	0.15693731517504494	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCTTATGTTGGATTGGCACGACAGATCGTCGGACTGTAGAACTCTGAAC	1141	0.15344085399719476	Illumina DpnII expression Sequencing Primer (100% over 26bp)
GTGTTGACTTTGTATACTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	1131	0.15209606123648317	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCAGATCATGAATTAGTAACTGATCGTCGGACTGTAGAACTCTGAACGTG	1039	0.13972396783793634	Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	1034	0.13905157145758054	No Hit
TCCTAAGGTGCTACTAAATGGAAGGATCTTATCAACGTCCATGAAGATCGT	1011	0.1359585481079438	No Hit
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	974	0.13098281489331087	Illumina RNA PCR Primer (100% over 34bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	965	0.12977250140867042	No Hit
GTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	891	0.1198210349794045	Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAACG	889	0.11955207642726218	Illumina DpnII expression Sequencing Primer (96% over 26bp)
TGCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	846	0.11376946755620224	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCGCGAAATGGATCATAATAAAATAGGATCGTCGGACTGTAGAACTCTGAA	804	0.10812133796121348	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATATCCGTTGCCGAGAGTCGTGTGGATCGTCGGACTGTAGAACTCTGAAC	803	0.10798685868514232	Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCTACCATCTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGT	799	0.10744894158085769	Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAATGAAATACCACAACCTACCCGATATGATCGTCGGACTGTAGAACTCT	787	0.10583519026800374	Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCTGAGCTACATCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	752	0.10112841560551311	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTAAGAATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAG	746	0.10032153994908614	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGGG	50	2.1827873E-11	45.043625	1
TGGTAGG	25	3.8672784E-5	45.043625	1
TGGAGCC	220	0.0	45.04362	1
TACTTAA	20	6.9981907E-4	45.04362	1
TGTATTT	85	0.0	45.04362	1
GCACCTC	55	1.8189894E-12	45.04362	1
TGGTGCG	180	0.0	45.04362	1
TGATCCT	30	2.1580581E-6	45.01938	4
ATGATCC	25	3.879595E-5	45.01938	3
AGCCCTT	70	0.0	45.01938	4
GGTCCCA	25	3.879595E-5	45.01938	5
GAGGTCC	25	3.879595E-5	45.01938	3
GGTAGGG	25	3.879595E-5	45.01938	2
GTGCATC	20	7.0167787E-4	45.019375	3
CTATGCC	20	7.0167787E-4	45.019375	2
CTGTCAG	20	7.0167787E-4	45.019375	3
GGAGCCG	150	0.0	45.019375	2
TTGGAGC	20	7.0167787E-4	45.019375	5
GAGAGCA	80	0.0	45.019375	3
GCGGGAC	55	1.8189894E-12	45.019375	3

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content