H3NFYBCXY_l01n02_smallrna_ctrl025h_rep2_shoot.3520000006bfdb.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3NFYBCXY_l01n02_smallrna_ctrl025h_rep2_shoot.3520000006bfdb.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24007671
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	8858875	36.9001849450536	Illumina RNA PCR Primer (100% over 46bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAA	491606	2.0477038359947537	Illumina RNA PCR Primer (97% over 45bp)
GGGGAATGAAGCCTGGTCCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	388313	1.6174538546450425	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	260931	1.08686511073898	Illumina RNA PCR Primer (100% over 38bp)
GATCCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	245269	1.021627628935768	Illumina RNA PCR Primer (100% over 45bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGATCGTCGG	197599	0.8230660941663187	No Hit
GCCAGATCATGAATTAGTAACTGGATCGTCGGACTGTAGAACTCTGAACGT	153563	0.6396413879547083	Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGATCGTCG	107903	0.44945217718120173	No Hit
GCCTGACCTGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	107456	0.4475902722925518	Illumina RNA PCR Primer (100% over 34bp)
AATTCCACGGTCGAACTACCGATCGTCGGACTGTAGAACTCTGAACGTGTA	98305	0.4094732887667446	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCTCGGTGTAAACGAGTTGCTCTACCGAACTGAGCTATAGCCCTGATCGTC	97726	0.40706155961567453	No Hit
GGAATGAAGCCTGGTCCGAGAGATCGTCGGACTGTAGAACTCTGAACGTGT	97107	0.40448321705174983	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	96090	0.4002470710299221	Illumina RNA PCR Primer (100% over 34bp)
GCCAGATCATGAATTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	78833	0.3283658793891336	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GTGCTCACTCTCTTCTGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGT	78777	0.3281326206111372	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGCCGGCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	75203	0.3132457121725802	Illumina RNA PCR Primer (100% over 35bp)
GGCTGCGCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	72419	0.3016494186379012	Illumina RNA PCR Primer (100% over 34bp)
TCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGA	68960	0.28724152376130113	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	67879	0.282738796278906	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCCGACGGGTTCGGGGCCGGGACCCCCGAGCGATCGTCGGACTGTAGAACT	63445	0.2642696994639755	No Hit
AGGGTCGAGCAGGTCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT	61479	0.25608065022217275	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GATCGTTGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	52954	0.22057116660753975	Illumina RNA PCR Primer (97% over 46bp)
GGGAATGAAGCCTGGTCCGAAGATCGTCGGACTGTAGAACTCTGAACGTGT	51262	0.21352341924379087	Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGGCCTCGGGCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATCGTC	50005	0.2082875927448356	No Hit
GCCGCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	49625	0.20670476532271706	Illumina RNA PCR Primer (100% over 38bp)
GGGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	46473	0.1935756283897759	Illumina RNA PCR Primer (100% over 38bp)
GGCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	46299	0.19285086004385849	Illumina RNA PCR Primer (100% over 34bp)
GATCGTCGGACCTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA	45138	0.18801490573575422	Illumina RNA PCR Primer (100% over 40bp)
ACGAATCCGTGCGACGCGGGGCTGGATGATCGTCGGACTGTAGAACTCTGA	43891	0.18282073259001258	Illumina DpnII expression Sequencing Primer (100% over 24bp)
GCCGCCGGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	43315	0.18042149944490657	Illumina RNA PCR Primer (100% over 35bp)
GGCAATTATCCTAACCGTTGGACTACAGACGCGATCGTCGGACTGTAGAAC	43210	0.17998413923616333	No Hit
GCCCGCCGCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	43172	0.17982585649395144	Illumina RNA PCR Primer (100% over 35bp)
GCCCCCGCCGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG	42492	0.17699342847542354	Illumina RNA PCR Primer (100% over 34bp)
AGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTCT	40828	0.1700623105006729	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCCGCCCGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	39282	0.16362270209384325	Illumina RNA PCR Primer (100% over 37bp)
GGCCCCGCTTCCGACTCACGGAATAAGTAAAATAACGGATCGTCGGACTGT	38905	0.16205237067768882	No Hit
GGCCGCCCGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	38716	0.1612651223019509	Illumina RNA PCR Primer (100% over 36bp)
AGTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAA	37750	0.1572414083815127	No Hit
AATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTC	36536	0.15218469130137613	Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTAGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT	35650	0.1484942042066471	Illumina RNA PCR Primer (97% over 46bp)
TCCCGGTCCGTCCCCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATC	35563	0.14813182003368838	Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGGCGGGAATACTCACCACTATACTACAACGACGATCGTCGGACTGTAGAA	35524	0.1479693719561552	No Hit
GCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	34690	0.14449548229813713	Illumina RNA PCR Primer (100% over 36bp)
GCCCGCCGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	34073	0.14192547040485518	Illumina RNA PCR Primer (100% over 37bp)
AGCCCACATGAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	33544	0.13972200801985332	Illumina RNA PCR Primer (100% over 35bp)
CAGTTCAAGAAAGCCTGTGGAGATCGTCGGACTGTAGAACTCTGAACGTGT	33192	0.138255809986733	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACGAATCCGTGCGACGCGGGGCTGGAGATCGTCGGACTGTAGAACTCTGAA	32570	0.13566497141684422	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCCGCCGCCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG	32319	0.1346194722511817	Illumina RNA PCR Primer (100% over 36bp)
ACGAATCCGTGCGACGCGGGGCGATCGTCGGACTGTAGAACTCTGAACGTG	31673	0.1319286656335802	Illumina DpnII expression Sequencing Primer (96% over 29bp)
GGCCGCCGGGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	31621	0.13171206819686923	Illumina RNA PCR Primer (100% over 35bp)
GTCCTCTGCTCTACCAACTGAGCTATCCTGACGATCGTCGGACTGTAGAAC	30296	0.12619299889606117	No Hit
GGCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC	29225	0.12173192476687973	Illumina RNA PCR Primer (100% over 39bp)
GATTCACAATCCACTGCCTTGATCCACTTGGCTACATCCGCCGATCGTCGG	28635	0.11927437692727462	No Hit
GGCCGCCGGGGGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG	28605	0.11914941686763368	Illumina RNA PCR Primer (100% over 33bp)
AGCCCCCGGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG	28267	0.11774153352901244	Illumina RNA PCR Primer (100% over 37bp)
AACGTGGAATTCTCACCACTAAACTACAGCCACCGATCGTCGGACTGTAGA	28228	0.11757908545147924	No Hit
CTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCTGAA	27670	0.1152548283421578	Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGGACCAGCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT	27152	0.11309718464569095	Illumina RNA PCR Primer (100% over 35bp)
ACGAATCCGTGCGACGCGGGGCTGATCGTCGGACTGTAGAACTCTGAACGT	26458	0.11020644193266395	Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGGTCGAGCAGGTCGGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA	26067	0.1085777958220104	Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAGTATCCTAACCAGCTAGACTACAACGGAGATCGTCGGACTGTAGAACTC	26056	0.10853197713347539	Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGATCGTC	25715	0.10711159778889005	No Hit
GGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT	25616	0.10669922959207497	Illumina RNA PCR Primer (100% over 38bp)
ACGAATCCGTGCGACGCGGGGCTGGATCTCAGTGGATCGTGGCAGGATCGT	25592	0.10659926154436222	No Hit
GGGCGGTGCTCTGACCAATTGAACTACAATCCCGATCGTCGGACTGTAGAA	25074	0.10444161784789537	No Hit
GTCCCGATCTGCACCAAGCGAGATCGTCGGACTGTAGAACTCTGAACGTGT	25037	0.10428750044100486	Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATTCTCACAACGACAGGGTCTACTCGATAGATCGTCGGACTGTAGAACTCT	24125	0.10048871462792039	Illumina DpnII expression Sequencing Primer (100% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCCGTAA	52900	44.654457	45
GCCGTAT	930695	44.48415	45
TAGTAAC	22760	44.16704	15
GTAACTG	22130	44.163918	17
TTAGTAA	23340	43.936222	14
ATTAGTA	23775	43.63388	13
AGTAACT	22560	43.42187	16
GCTCACT	11695	43.34582	3
TGCTCAC	11710	43.17964	2
AACTGGA	18185	43.117195	19
CGCCGTA	1022540	42.860542	44
CTCACTC	11840	42.85247	4
GCGTGAC	7000	42.796825	1
TCGCCGT	1025380	42.75323	43
GGTCGCC	1027870	42.735634	41
GTGACGC	6990	42.701725	3
TGGTCGC	1029055	42.673664	40
TGGCCTC	43050	42.600616	1
CCGTATC	10495	42.508076	45
GTGGTCG	1033495	42.506226	39

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content