FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l01n01_smallrna_heat_1h_rep2_shoot.3510000006c02d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l01n01_smallrna_heat_1h_rep2_shoot.3510000006c02d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32724954
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACCAGGCTTCATTCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT7166972.190062665939882RNA PCR Primer, Index 1 (100% over 30bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT3919901.1978320886257012No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTCAAGGCGGAAGCTGC3867331.181767894922022No Hit
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTC2593390.7924808694918257No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATGCC2264260.691906243779594RNA PCR Primer, Index 8 (100% over 51bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATCCGGCCG2259640.6904944770892574No Hit
CAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCC2117240.6469802829974949RNA PCR Primer, Index 1 (100% over 27bp)
AGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCA2082090.6362392442171194RNA PCR Primer, Index 1 (100% over 28bp)
CCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTC2063150.6304516119411505RNA PCR Primer, Index 1 (100% over 26bp)
TAAATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCCTA2025790.6190352475361768No Hit
CCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT1868600.5710015665721028RNA PCR Primer, Index 8 (100% over 43bp)
GCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1802650.5508487498561495RNA PCR Primer, Index 1 (100% over 29bp)
TCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACT1562300.4774032684660152RNA PCR Primer, Index 1 (100% over 25bp)
CTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAG1557340.4758876055257404RNA PCR Primer, Index 1 (100% over 29bp)
CACACGACTCTCGGCAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1530850.4677928653467321RNA PCR Primer, Index 1 (100% over 32bp)
TAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC1523590.4655743748333458RNA PCR Primer, Index 5 (100% over 35bp)
CAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA1479290.4520373046208102RNA PCR Primer, Index 1 (100% over 28bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTTGGAATTCTCGGGTGCCAAGG1466660.44817786451281183Illumina Small RNA Adapter 2 (100% over 21bp)
TATCGAGTAGACCCTGTCGTTGTGAGATGGAATTCTCGGGTGCCAAGGAAC1436410.4389341540403693RNA PCR Primer, Index 1 (100% over 24bp)
TCTCGGACCAGGCTTCATTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1308740.39992111218857634RNA PCR Primer, Index 1 (100% over 30bp)
ATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAAC1223370.37383398613791785RNA PCR Primer, Index 1 (100% over 24bp)
TCGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT1162100.3551112707446434RNA PCR Primer, Index 8 (100% over 41bp)
GCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC1150420.3515421289820606RNA PCR Primer, Index 8 (100% over 42bp)
TTCGGACCAGGCTTCATTCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1105680.3378706047990167RNA PCR Primer, Index 1 (100% over 30bp)
GGCGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT1047060.3199576690008487RNA PCR Primer, Index 8 (100% over 43bp)
GGTAGTTCGACCGTGGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1005570.30727927073633166RNA PCR Primer, Index 1 (100% over 31bp)
GCCGGCGGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA972260.2971004940144454RNA PCR Primer, Index 8 (100% over 39bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC934220.2854763371095953RNA PCR Primer, Index 5 (100% over 35bp)
GGGGATATGGCGAAATCGGTAGACGCTACGGACTTTGGAATTCTCGGGTGC923080.2820722070380909No Hit
CCCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA902030.2756398068580937RNA PCR Primer, Index 8 (100% over 39bp)
GTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGA828290.25310654370973296RNA PCR Primer, Index 8 (100% over 39bp)
GATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAA807780.24683915522081407RNA PCR Primer, Index 1 (100% over 23bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTGGAATTCTCGGGTGCCAAGGA761020.23255036508225496RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATT756340.23112026375957628No Hit
GGGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA739160.2258704473656403RNA PCR Primer, Index 8 (100% over 40bp)
TATCGAGTAGACCCTGTCGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA737370.22532346416743626RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA734850.22455340960907078RNA PCR Primer, Index 8 (100% over 40bp)
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGTTTACACCGAGATGGAATT721000.22032116531011778No Hit
TATCGAGTAGACCCTGTCGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA705910.21571000527609605RNA PCR Primer, Index 1 (100% over 28bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCT691160.2112027414920125RNA PCR Primer, Index 8 (100% over 43bp)
TATCGAGTAGACCCTGTCGTTGTGAGTGGAATTCTCGGGTGCCAAGGAACT691010.2111569049111574RNA PCR Primer, Index 1 (100% over 25bp)
TATCGAGTAGACCCTGTCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG671990.20534482645873237RNA PCR Primer, Index 1 (100% over 29bp)
CGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC651980.1992302265726638RNA PCR Primer, Index 5 (100% over 35bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTCGTATG638230.19502853999428083RNA PCR Primer, Index 8 (100% over 49bp)
AGGAATGATGAATCAATTAGATGTACGAGGTTACCTTATGAGCTAAAATTT611720.18692768827115847No Hit
GGCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT592230.18097198853205418RNA PCR Primer, Index 8 (100% over 41bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGGAATTCTCGGGTGCCAA578150.17666946147579No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTTGGAATTCTCGGGTGCCA578070.17664501529933396No Hit
GAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA567090.1732897775807416RNA PCR Primer, Index 1 (100% over 34bp)
CAGGCTTTCTTGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA560860.17138603158922697RNA PCR Primer, Index 1 (100% over 34bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCGTGGAATTCT528790.1615861706024094No Hit
TATCGAGTAGACCCTGTCGTTGTGAGAATGGAATTCTCGGGTGCCAAGGAA527400.1611614182864856RNA PCR Primer, Index 1 (100% over 23bp)
TCCTAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCC516490.15782757097229227RNA PCR Primer, Index 1 (100% over 27bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTGGAATTCTCGGGTGCCAAG513070.1567824969287963No Hit
GTTTAAATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATC511290.15623856950264928No Hit
CCAGGTCAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA508370.15534628406200357RNA PCR Primer, Index 8 (100% over 40bp)
TCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGG505310.15441121781255981No Hit
CACACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAAG502350.1535067092836861No Hit
CACACGACTCTCGGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC489320.14952503829340755RNA PCR Primer, Index 1 (100% over 33bp)
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTTGGAATTCTCGGGTGCCA483910.1478718656105674No Hit
CATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATCCGGCCGAGGGCAC482100.14731877086824935No Hit
TATCGAGTAGACCCTGTCGTTGTGAGAATTCTTGGAATTCTCGGGTGCCAA481730.14720570730214014No Hit
CGGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATC474970.14514000539160424RNA PCR Primer, Index 8 (100% over 42bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC474550.14501166296520998RNA PCR Primer, Index 8 (100% over 44bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGGAATTCTCGGGTGCCA473040.1445502413846021No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTTGGAATTCTCGGGTGCC465270.1421759064963086No Hit
ATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATCCGGCCGAGGGCACG448690.13710943642579299No Hit
CCCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA435250.13300247878117719RNA PCR Primer, Index 8 (100% over 40bp)
AGGATCATTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT435170.13297803260472116RNA PCR Primer, Index 8 (100% over 41bp)
GTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACTGGAAT427080.13050591301060346No Hit
TCTGTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAA425400.1299925433050265RNA PCR Primer, Index 8 (100% over 40bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATGGAATTCTCGGGTGC417500.12757848337999192No Hit
CAACATAGGTCATCGAAAGGATCTCGGACGACTCACCAAAGCACGAAAGCC409590.12516136768290034No Hit
CACACGACTCTCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT405940.12404601088209322RNA PCR Primer, Index 8 (100% over 36bp)
AATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCCTAAC400680.12243867478010817No Hit
CCCCGGCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAAT399530.12208726099355251RNA PCR Primer, Index 8 (100% over 41bp)
AGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG397730.12153722202329145RNA PCR Primer, Index 1 (100% over 29bp)
CACACGACTCTCGGCAACGGATATCTGGAATTCTCGGGTGCCAAGGAACTC397190.12137221033221314RNA PCR Primer, Index 1 (100% over 26bp)
GGCGGACTGCTCGAGCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA390320.11927289492905017RNA PCR Primer, Index 1 (100% over 32bp)
GGCGGACTGCTCGAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA379910.11609183621770712RNA PCR Primer, Index 1 (100% over 34bp)
TTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTTGAATCTC372510.11383056489552286RNA PCR Primer, Index 8 (100% over 44bp)
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATCCGGCCGA369610.11294439099899117No Hit
CCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT366730.1120643286465735RNA PCR Primer, Index 1 (100% over 30bp)
AGATCCAGCCCCGCGTCGCACGGATTCGTTGGAATTCTCGGGTGCCAAGGA365810.11178319761732897RNA PCR Primer, Index 1 (100% over 22bp)
CCGACCTGCTCGACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA358740.10962276677302586RNA PCR Primer, Index 1 (100% over 34bp)
GCCGTCGGCGCAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT357140.10913384324390495RNA PCR Primer, Index 8 (100% over 36bp)
ATGCAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACT357010.10909411820716386RNA PCR Primer, Index 1 (100% over 25bp)
TAACGAACGAACGATTTGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT354530.10833628673702642RNA PCR Primer, Index 1 (100% over 30bp)
CGTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCGTGGAATTC352340.10766707265654216No Hit
GGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGTGAATCTGGAATTCT344440.10525301273150758No Hit
CACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAAGGA338660.10348677648255823RNA PCR Primer, Index 1 (100% over 22bp)
GTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATG334430.10219418490244479No Hit
GGGGGACGGACCGGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC332470.10159525357927165RNA PCR Primer, Index 5 (100% over 35bp)
TGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCTC332420.10157997471898661No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCTGGAATTCTCGGGTGCCAAGGA328790.1004707294622935RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAG1133600.044.841831
TCAGAGC554000.044.7143720
GGTCAGA557250.044.64757518
TAGTTCA603750.044.6444368
AGTTCAA601650.044.6210569
CAGAGCA547600.044.6148121
TCAATTG563000.044.59145412
GTTCAAT602650.044.5657410
TCGAGTA1140900.044.513293
TGGTCAG559200.044.51199717
GCCCTGT418500.044.4636730
CACACGA572150.044.446221
TAGACCC1143450.044.3887188
TTCAATT567600.044.32123611
CTGTCAA409150.044.31410633
GTAGACC1147600.044.27917
AGTAGAC1147350.044.2733356
CGAGTAG1149000.044.2288634
TGTCGTT1116750.044.21555315
CCGCCCT481550.044.20332328
Produced by FastQC (version 0.11.2)