FastQCFastQC Report
Thu 27 Oct 2016
H3NFYBCXY_l01n01_smallrna_ctrl025h_rep3_shoot.3510000006bff8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3NFYBCXY_l01n01_smallrna_ctrl025h_rep3_shoot.3510000006bff8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743609
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC25084033.732781609690036RNA PCR Primer, Index 6 (100% over 51bp)
TATCGAGTAGACCCTGTCGTTGTGAGATGGAATTCTCGGGTGCCAAGGAAC132401.7805056151821725RNA PCR Primer, Index 1 (100% over 24bp)
TAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC81181.0917027631456855RNA PCR Primer, Index 5 (97% over 35bp)
CAGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCA73950.994474246546236RNA PCR Primer, Index 1 (100% over 28bp)
TATCGAGTAGACCCTGTCGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA62550.8411678718251124RNA PCR Primer, Index 1 (100% over 28bp)
TATCGAGTAGACCCTGTCGTTGTGAGTGGAATTCTCGGGTGCCAAGGAACT46010.618739149203412RNA PCR Primer, Index 1 (100% over 25bp)
GGGGACGTAGCTCATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC32710.43988171202876786RNA PCR Primer, Index 5 (97% over 35bp)
AGCGTGGAGGTTCGAGTCCTCTTCAAGGCACCATGGAATTCTCGGGTGCCA27530.3702214470239064No Hit
TATCGAGTAGACCCTGTCGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG26490.3562356023125056RNA PCR Primer, Index 1 (100% over 29bp)
CACACGACTCTCGGCAACGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT25980.34937715923287643RNA PCR Primer, Index 1 (100% over 30bp)
TATCGAGTAGACCCTGTCGTTGTGATGGAATTCTCGGGTGCCAAGGAACTC22180.2982750343258352RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC20200.2716481376637453RNA PCR Primer, Index 5 (97% over 35bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGATGGAATTCTCGGGTGC19440.26142771268233705No Hit
TATCGAGTAGACCCTGTCGTTGTGAGAATGGAATTCTCGGGTGCCAAGGAA19370.26048635774983897RNA PCR Primer, Index 1 (100% over 23bp)
CTGGGCGTCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA18630.25053489132057305RNA PCR Primer, Index 6 (100% over 38bp)
ATATCGGGTAGGTTGTGGTATTTCATTGCTACAATGGAATTCTCGGGTGCC17630.23708696371345694No Hit
TATTCTGGTGTCCTAGGCGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT16730.22498382886705243RNA PCR Primer, Index 1 (100% over 30bp)
CACACGACTCTCGGCAACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA16510.22202528479348688RNA PCR Primer, Index 1 (100% over 32bp)
CCTGGGCGTCACGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA16190.21772194795920977RNA PCR Primer, Index 6 (100% over 37bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCAAATATCTCGTATGCC15760.21193933908814985RNA PCR Primer, Index 6 (98% over 51bp)
GGGGGTGTAGCTCATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC15050.20239131048709738RNA PCR Primer, Index 5 (97% over 35bp)
TAATTCATGATCTGGCATGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA13070.17576441382500751RNA PCR Primer, Index 1 (100% over 32bp)
TATCGAGTAGACCCTGTCGTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA12590.16930940857359178RNA PCR Primer, Index 1 (100% over 28bp)
TGTCGTGCCAATCCAACATAAGCCTTGGAATTCTCGGGTGCCAAGGAACTC12230.16446815463502998RNA PCR Primer, Index 1 (100% over 26bp)
GGGGATGTAGCTCAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC12030.16177856911360675RNA PCR Primer, Index 5 (97% over 35bp)
AGGGATGTAGCGCAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC11720.15760971155540077RNA PCR Primer, Index 5 (97% over 35bp)
AGTTACTAATTCATGATCTGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG11300.15196158196041198RNA PCR Primer, Index 1 (100% over 29bp)
AGTATACAAAGTCAACACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC11090.1491375171629176RNA PCR Primer, Index 1 (100% over 33bp)
NGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC10940.1471203280218502RNA PCR Primer, Index 6 (98% over 51bp)
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGTTTACACCGAGATGGAATT10560.14201011553114606No Hit
TTCATGGACGTTGATAAGATCCTTCCATTTAGTAGCACCTTAGGATGGAAT10380.13958948856186518No Hit
GTGAACCATCGAGTCTTTGAACGCAAGTTGCGCCCGAGGCCATGGAATTCT9870.13273104548223597No Hit
GCGCCGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT9640.12963802213259926RNA PCR Primer, Index 6 (100% over 39bp)
TATCGAGTAGACCCTGTCGTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC9190.12358645470939701RNA PCR Primer, Index 1 (100% over 27bp)
ATATCGGGTAGGTTGTGGTATTTCATTGCTGGAATTCTCGGGTGCCAAGGA9040.12156926556832959RNA PCR Primer, Index 1 (100% over 22bp)
TAATTCATGATCTGGCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG8930.12008999353154681RNA PCR Primer, Index 6 (100% over 34bp)
CTATTTTATTATGATCCATTTCGCGATGGAATTCTCGGGTGCCAAGGAACT8890.11955207642726218RNA PCR Primer, Index 1 (100% over 25bp)
TTCATGGACGTTGATAAGATCCTTCCATTTAGTAGCACCTTAGGATGGCAT8890.11955207642726218No Hit
CACACGACTCTCGGCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC8860.1191486385990487RNA PCR Primer, Index 1 (100% over 33bp)
TATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATGGAATTCTCGGGTGC8290.11148331986299252No Hit
CACACGACTCTCGGCAACGGATATCTGGAATTCTCGGGTGCCAAGGAACTC8090.1087937343415693RNA PCR Primer, Index 1 (100% over 26bp)
GGGGATGTAGCTCAGATGGTAGATGGAATTCTCGGGTGCCAAGGAACTCCA7990.10744894158085769RNA PCR Primer, Index 1 (100% over 28bp)
GGGGATGTAGCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC7710.10368352185086517RNA PCR Primer, Index 5 (97% over 35bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCAA7590.10206977053801125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATAC1150.045.1588631
GTCGATA406.6011125E-945.158861
ATCAGCC206.9106446E-445.158861
AGGGCTA2400.045.158861
ATCACGC253.888991E-545.00094628
GTCACGG253.888991E-545.00094629
ACACGCG302.1641517E-645.00094222
GACACGC302.1641517E-645.00094221
AGAACCG551.8189894E-1245.00094223
CCGTATA453.8380676E-1045.00094227
GAGCAAC1600.045.00094223
ACCGTAT551.8189894E-1245.00094226
GTATTAT302.165154E-644.9979175
GGGCTAT2400.044.9979172
CTATAGC2350.044.9979135
GCTATAG2350.044.9979134
TAGTTCG750.044.9979133
GGGACGT3300.044.9979132
ATATAGC406.8121153E-944.9979135
GATATAG406.8121153E-944.9979134
Produced by FastQC (version 0.11.2)