FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n02_negcon15_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n02_negcon15_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTTCGCTGATCAAGGTTACGGTGTAGGTGATCTTCCCGCCTTCGACG50.4716981132075472No Hit
GGGTTTGATGGCGGAAAGCTGGCTCGTCAGCATGTCGCGGATCTGCTCAC40.37735849056603776No Hit
AGGTGGCGCAATGGTGTTGATCCAATCCTGCAGTCCCAAAGCACAGAGCA40.37735849056603776No Hit
CAATCCATGTCGGCCACCGAGGTGCCGCTGTCGATGGCCGTGATGTAGCG40.37735849056603776No Hit
AGGCCCTGGTGAAGCCCCACGTCGCGCTGCGGGTTCTGGGCAACATCATG30.2830188679245283No Hit
GGGCGCTTGTTTCCGGAGCACGTCGGCGCCCGAAAGTGCTTCGTCAACAT30.2830188679245283No Hit
TCCGATTAGTTCTCTTCTTTTTTGATCCAGCTCACCAAACTTATCAAAAT30.2830188679245283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2830188679245283No Hit
AGCCCAGGTCGCGCAACTCAATTGCCCCGTTCACCTTCTCGATGTGCACG30.2830188679245283No Hit
CGTTTGCGCACTACCTGCACGTGGTGCAAGACCCGTACTACTACGAAATC30.2830188679245283No Hit
CTTTCGGGAAGACTCTACAGACAACCATTTGCCCTGTATCTAATTGGTTT30.2830188679245283No Hit
TGAACGCTGTCTCTTTCCCCGACATTCTGGTTCGCGTGGCCAAGCTGCCG30.2830188679245283No Hit
GATGCAGCCATACCAATACAAATGGTTGATACCTTTGGTTTAATAAATTG30.2830188679245283No Hit
GACCTGCACCCCGCCGATATCGCCTACATCCTCGAAGCCTTGCCCCTCGA30.2830188679245283No Hit
GCACCTGCTCCATCTCCAAACAAAACAGCTGTGTTGCGGTCATCCCAGTC30.2830188679245283No Hit
ACTTAAAGCATGGCTGACAAGAACACTTGAAATCCCTCCAGACGTCATGA30.2830188679245283No Hit
CAGACAGTGACCCCCGTCCTTCGGGTAACTAGTGCTTCCGCCAGTTTTCC30.2830188679245283No Hit
GTGTTGCCCAACGGCATGAACATCGAGTGGACCGACCTGAGCTACCAGCA20.18867924528301888No Hit
GGCCGTGCCGGGCGCAGCCATGACGGCGACTGCCGTGGCGCGGATATCCG20.18867924528301888No Hit
TGATGCGGCAGTCGATTATGCGAAGGAGCGCAAGCAATTCGGCAAACCAA20.18867924528301888No Hit
TGCCAGTAGCGGTCGAGCATGACATCGCCGACCACCAGTACCGGGGCTTG20.18867924528301888No Hit
GGCACCCCTCGCTACCGCGATTGCCAGCGTGCAAGCCACGCCGGGCGTGC20.18867924528301888No Hit
GCACTCGACGTTCAAGAAGGGGGCATTTCAGATGGACACTTCGCCGGCGG20.18867924528301888No Hit
GTTATTACAGTTCCTGACGATCCCGGTAAGCGAGATGTTCCGCGACCCGT20.18867924528301888No Hit
CATATCATCTTCTTATCCTTTTTTATAGCGACGAATCATATAGTGGATGA20.18867924528301888No Hit
AGGAATAAAGAGCACAGGCGCTTTGGCCTTCCGCGCCATTCGATATCTCT20.18867924528301888No Hit
ACCCAGATGCAGGTCATGTAAATGCGGTCGTTGATGCGCAGCAGTAAATT20.18867924528301888No Hit
GTTTCTCTTCTGGCTGCCGAAGGTGGAAGTACGCAGATAGCTCAACGGTC20.18867924528301888No Hit
CCGTGGAGGTGCCCGGCGGGTAATAGTTGGGAATCCAGTAGTAGTTCCAC20.18867924528301888No Hit
ACTCAAATCCACATAGCCATGCAGGCGGCATTCTGAGAGAAATATCTGAA20.18867924528301888No Hit
AGGTAATTCTCCGGCCATGGGCGGCCAGTGCACCTTGCTGGCGCGAGTGG20.18867924528301888No Hit
CTGCAAGCCGGTCATGGAAGCCATCCGCAGCAATCAGCCGCAACGTGGTC20.18867924528301888No Hit
CGTAAGGGGGCACCACATTACCGACGTTACGGGTAACGAACAGGTCGCCG20.18867924528301888No Hit
GCCCGATGGTCACTGAAAGTGGGTAGAATGCGGTTTTTTTCCAGTTGCGG20.18867924528301888No Hit
CACAAGCACCCACACGAATTGCTTGATTCGATTTGTTAAAGAGCGTTTGG20.18867924528301888No Hit
AAACAAGGACTCGGCAATTCGGCTAAGCATTCGCGGCTACCTCCTGCGCG20.18867924528301888No Hit
TGTGCAGGGTCTTGCCCTGCTGCTTGAACTCTTCGATTTCGACGGTGATC20.18867924528301888No Hit
GTTCGAGCGAGGCTTGACCGATGTCTCGGCCACGTACCAGCCGCAACCCG20.18867924528301888No Hit
AAACGGCGTCGTCGAAGTCGCGCGCGTCCTCGCCGTCGATGGTCACGAAG20.18867924528301888No Hit
GAAGAAGCCGTCGTTGGCGATGGGTGGGCCGCCGGGCGTGGCCGGCTGTG20.18867924528301888No Hit
CAGCTACACAAGATATTCACTCTGTTTTTCCAGCAAAAGCACTCAGGCAG20.18867924528301888No Hit
ATCCGCTCCCGCACCCAGGCGAGGTCATCGGCATGGATGTGCGCCTCGGT20.18867924528301888No Hit
TCATCAACGAGGTCAGGTCGAACCCCGGCGTCGACTTCGTCGAGCTGACC20.18867924528301888No Hit
TTTTTGTACTGAGGGCCGAGAGAAGGCGGCCAGCCGGTTGATGCGAGAGA20.18867924528301888No Hit
GGAAAAACTGCTTCCAGCCCTGCTCGGCAGCGGCCTGGCTCGGCGCCTGG20.18867924528301888No Hit
GACTATTGACCGCCCTTGCGTTGTTCATTGATGCCGGCGTACACCTCTTC20.18867924528301888No Hit
CTTTCGCTACGCCTCCCCTATTCGGTTAAGCTCGCCACTGAATATAAGTC20.18867924528301888No Hit
ATCTTGAGGGAGATTTCCTGACCGTCGCGGGCGTGGCCGTTGAGGTATTG20.18867924528301888No Hit
GGTCTGGGTCATGTCCAGCACGCAGCCGGAGCCGTTGACCCGATCGATGG20.18867924528301888No Hit
CATAAATATCGTGCATGAGTACGGTTTTGCCATCTGCTGCATGTGAGAGA20.18867924528301888No Hit
GGGGCTACGAGCCTAGAAACTCCAAGCGCGCATTGCTTGGTTCTCCACCC20.18867924528301888No Hit
CTGCCTGCAGTGGGTTGGAAATCCACGCGCCAAATGTTGCCTCCCACATC20.18867924528301888No Hit
GTACTTTCCCTTTACGGAATCCAGGGATTGATACTTGCTTAACTACTTTC20.18867924528301888No Hit
ATTCCCTTATATGGAGCTAGCGGATAAATCGCTTTCTGATGAGGAAGTAA20.18867924528301888No Hit
GTAGAGAGGCGGAGCTGATCAGCGCACCCTGCAGGGGTTTGTCGTTCTCG20.18867924528301888No Hit
ACCTTCGACTTCTATGCCGGCTTCATCATCGGCATCAAGGCCTTCACCGC20.18867924528301888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)