FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n02_negcon07_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n02_negcon07_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4989
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCAGGACGGCCCGGACGATCCAGTTTGTCCAGGGCTGGCATTCCTGCA80.16035277610743637No Hit
GGCCAGCACGCTGGCGCGCAGCTTGTTGTCCGGGTGGTTGTAGGCGCGGC80.16035277610743637No Hit
TCGATAATCAATGCCAGGCTGCGCGTGCCGGCCGGAGCGCCTGCCCACGC80.16035277610743637No Hit
CATCAACTCGCCCTTTGGCCCATCGCGGCTGGTATTTGCGCAGCAGACCG70.14030867909400682No Hit
GCTTGCCGAGGTAGGCCGCAGTGGAGTCTGGCGTGGTGCGCAGGTCGAGT60.12026458208057728No Hit
GATCACCACTGCCGACGCAGCGCTTCTTCGTGCGAAGGAAGGCGGCCGCG60.12026458208057728No Hit
CAGCAACGGGGACTGGGCACCGAACAGCAGTTTCTGCACCGCTTGGTCAT60.12026458208057728No Hit
GTATGGGAACGATTGATCGAGAAGGTTATTGGTTCGTTGTGGCCAGCACT60.12026458208057728No Hit
GTATGCTGCGCAACCTGTATCACCTGCTGGCAGCGTAGCGTCGGCGCGAA60.12026458208057728No Hit
AATAAACCCAGTCCACGATATCGCCCTGCGGCGAATACCCGTTCACCATC60.12026458208057728No Hit
GAATCAGACCTCCACCCCTAGGAGGCACCCTGTATGCGCAATCGACTGCT60.12026458208057728No Hit
GCCAACTACTGGGTCGATGTGGTGCTGAACGGGTCGGCGTCGGTCAGCGT50.10022048506714773No Hit
CCGGTTCACAAGGCGGATCAACGTCGACTTGCCGGAGCCCGACAGGCCCA50.10022048506714773No Hit
ACCAAGCGGTTGCCGATTGTTTGAGGCGGGCCAGCCCTGCACGTAACGCT50.10022048506714773No Hit
CCTCAAGGCACAGATCACGGAAGACATGAAGGCCGCCATGCGTGCCAAGG50.10022048506714773No Hit
GTCCTGATGTACCGAGTGCAAGCCCTTTTGCCACTGGATTTTTAATTCGA50.10022048506714773No Hit
GGTCGCATCCTTGATCGTTTTCAAGTTGAAGATTCGCTTGAATCCGGAGA50.10022048506714773No Hit
CGGCTTGCCGCGTCTTTTTACGTTTGAGCCCGAACCCTGCCGCCACCTCG50.10022048506714773No Hit
GCGATGCACTGCACCGTTTGCTACTCGACAACCTGACCACCGCGACCATC50.10022048506714773No Hit
ACCTGGTGCATGACGACCCGCAGCGCTGTCGCGACGCCGGCATGGACGCC50.10022048506714773No Hit
ACCCGCAGGTCGACTGGTCGGCCATCGACGACGTGATCTTCGGCTGCGCC50.10022048506714773No Hit
GTGCAGGAATATTAACCTGCTTCCCATCAGCTACGCATCTCTGCCTCGCC50.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)