FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n02_negcon06_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n02_negcon06_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGACGGGCTTCACCTTCGTCGGCTTCTTCACGCCGATCCGAGACTTGG80.17323516673884798No Hit
CATATGTTCATTGTAAGAAATAATAGGATTAAAGTCTATGATGTAATTGT70.151580770896492No Hit
AAGGTGTGGTATGCGCAGACTCACCCGGAACTGTTCGACCAGCGGAGTGT70.151580770896492No Hit
TGATAAAGCGCTCCATTAATTCCTGAAAAGCAGATGCTGACTCAGCTTCT70.151580770896492No Hit
GCACGATCCCCGCCACCACCGATCGTTCGGCGTACCTCGCGTGGAGCCTG60.12992637505413598No Hit
GTCCACCTTGAACTGGCGGATCAGCTCCACCGTCGCCTCGCCGGTGACGC60.12992637505413598No Hit
GCATTGGGATGAGCTGGCCGACCGTCTGATCCCCTACGTGACGGCGCTCG60.12992637505413598No Hit
GCTCGTCACAGGGATGAGGAAGGTGGAAATGGCTGCAGCCTAACCCTTTA60.12992637505413598No Hit
CTTCCTATAAGCGTTCTTGGACTTCAATTTAGGTGTTGCCGACAACGCAC50.10827197921178No Hit
ATAGAACACCAGTGCGACAACCGCAGCGATGCTTGCCGCGGCCAACAGGA50.10827197921178No Hit
ATCAAGGCGAAATCATCGAGTGTCCCGACCCTATGCATTCTCACCGCAGA50.10827197921178No Hit
CATCATCTCTGATCCACTTGGGGTAGAACATAGGATTCTTGCGGAACGAT50.10827197921178No Hit
CCCGATCACCATGCACTCCAGCAACGAATTGCTCGCCAGCCGGTTGGCGC50.10827197921178No Hit
AGTTTGTATGAAGGGATTTCGCGTCTGCAACTGATTGCTGATACACCTCA50.10827197921178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)