FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n02_Tr3_NCI3a_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n02_Tr3_NCI3a_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGATTGACCGAACTGGAATTTTTTGATTACCGGGTTCACGGTGTCCTA80.18198362147406735No Hit
GCACAAGATTGAGCACACCCGCACGCGCGGCAGAGGTGGCCACCATGTGC70.15923566878980894No Hit
GTGTGATGCCGCGATCCCTGGTCGTCCCGGCCGTTGCGCTGACCCTCGCC70.15923566878980894No Hit
GGAGACATCCATGGCTGGTGAGGTTTTCGACGCGCGACCGCGCAACGGTG70.15923566878980894No Hit
GTTTGCGGACTTCGTGGAGTCGACACCGGCGCCGTTCCAGGCCCTCGTCA70.15923566878980894No Hit
GCGCCACCACGAGCACGAGGCTGACGGTGGAAAGCCGTTCGACGTCGCGC70.15923566878980894No Hit
GCGACAGATCTCGTAGCCGCCCTCCTCGCCTGACCGCGCGTGCCGATGTA70.15923566878980894No Hit
ATCTTGAGCTCCTGCACCGGCAGGTTGCGCAGGTAGGCCAGCGACGAGAA60.13648771610555052No Hit
CCTCCACGCCGGTGCTTGATCGCATCCGTGATACCGGCACCGTCCGCGTC60.13648771610555052No Hit
AGAATGCGTTTCTGTTGAAACTTCAGCAGGAGCGCTTCTCATGGATGCCG60.13648771610555052No Hit
GTCATCATCCTGGCAATCGCCATCTACGCCTTCAGGGCGCTGAAGACGCC60.13648771610555052No Hit
ACCTGAATCTCCGCCGCCGCACGCGGCCAGGGCCACGGTGGCGAACAGCG60.13648771610555052No Hit
CCGCCCTGCAGGAAGCCGACGTTGCCGACCACGACGGTGCGGGCGCGGAA60.13648771610555052No Hit
GTACGGCACACCGGCCGCTTCGATCGCCTCTTCCTGCGCAACTTTGGCGG50.11373976342129208No Hit
ATCCCATTCGGCGTCGTATGGGCTGATTGGTAGCCTTTTTTCGATTTCGT50.11373976342129208No Hit
GTTCGGCACCTTGGCGGCATCGAGCTTCTGGATCCAGCCCACCTGGATCA50.11373976342129208No Hit
TAGCCAGCCTCTGTCGGCGAACTTCACGCGTGCCGAAGTGCTGCGTCGGC50.11373976342129208No Hit
CTCCGAACGTGCGGGTGATGTTGCTGCCCTTGACGATCGGGTCGGGCTTG50.11373976342129208No Hit
ATCTTCTGGGCGCCGTTCCCGCTGCTCGGCTTCGAGCCGTGGATGCTCTA50.11373976342129208No Hit
CTCGAGGACCTTCGCGGCCGTGCCGCGACCGGCGCCCGTCATGCCCGTCA50.11373976342129208No Hit
GTACGAGACGTGCAGGCCCGGGACGTCCCGGTTCTCCAGCTGCAGCTGGC50.11373976342129208No Hit
CCAGCAGCACCGCGCCAATCGTTTTCAGGTACGGCAGCGCAAGCAGCTTG50.11373976342129208No Hit
GGAGTAGAGTGGGGCACCATCCTCCTGTTCCGCACACGGACGCGTGCCCG50.11373976342129208No Hit
GACCGGGGCAGCGCGGACCTGATCGACCGCTACGTGCGACCGACGCTCGC50.11373976342129208No Hit
TCATAGCGCAGATCGACACGGGCATTGCCGGCAACCGGAACGCCAAGCAG50.11373976342129208No Hit
CCCGCTTCGCTGCGGCTGATTTTTTTGTGATTCCGAAAGACTTACGATGA50.11373976342129208No Hit
ACCCGGACCGGTGTCTTCAGCGCGGATGCCTGTGAGGCGTGCGCTGCGTC50.11373976342129208No Hit
GTCCTTGAGCGACCAGCCGGAGACGTCGACCGCGGTGTCGCCGATGTTGG50.11373976342129208No Hit
GTCCTGGTCGGCGCCCTCAAGCGCGTCCGGGCAGACGGCGGCTCGCTCGA50.11373976342129208No Hit
GGACGAAGATGACTTTCCGCCTGGACCGAAACACGAGGTCCGGATGTCCA50.11373976342129208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)