FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n02_133b_2_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n02_133b_2_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4549
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGCAATGAATCCCCGCAAAGCCTTTTTCGCCTGCCTGGAACGCTCCC90.19784568036931194No Hit
ACCTCCGAGCGCACGCCATCGTCGGGAACGCCGCCCGGGCACTCGAGCTG70.15387997362057595No Hit
GGGCTGGCCTTCGCGATCGACACCCAAGTCGCCTGTGACGATGCCGCGTA70.15387997362057595No Hit
GGTGTGCACATCCTGACGCAGCGTGGTGACAAACAGTTCGCGCATCTTGG60.13189712024620795No Hit
ATACGCCATCGCGGGCAAGCCCGCTCCCACAGGAAAATCTGTTTGCCTGA60.13189712024620795No Hit
GTTGAAAATCGTCCACCTGCTAATGGGCGCAGCAGCCTTGCTGCTGTCGT60.13189712024620795No Hit
TTGTTGGCCCTAAGTCTAAGATGAAATCGACGGCTTGCTTGATCATACAT60.13189712024620795No Hit
CCCTGATATGAAGTGAGGCTCGGAAAAACCGGGCCTCACTCATGGCTTGC50.10991426687183997No Hit
TCCCCATCCAGGCCGTCCCGCCCTTCGCCAGGAAGGTCGCCGCAGTCTCG50.10991426687183997No Hit
TCATTGGATATGTTGTCTATCACCGCTATGTGAAAAAACATCAGGTGAAA50.10991426687183997No Hit
GGGCACGCCCATTGATCACCCACAACCTGCGCCTGGCCGCTTGACCCTCA50.10991426687183997No Hit
CCGTAGACGCGTTCGACGTCGTACGGCGAGAACAGGTACATGTCCTCGTC50.10991426687183997No Hit
GCCCTTAACGATTGCGCCTTTGTCGTTAACCGAGACGTACTCGGAGAACG50.10991426687183997No Hit
ATATTAAAAGGTAAGCGTGTTGATAGTATTGCAAAAACATTGAAAGTGAG50.10991426687183997No Hit
GCCCATCACATCCGGTACAAACTCCACCGGTTTGCCGGCAATCACGATGC50.10991426687183997No Hit
CTTCCAACTGGTGCCGCGCAACACCCAGGCCACCGTCGATGCGCGCTTCT50.10991426687183997No Hit
GGCTACAGCTTCATTCACGGTACGTTCAAGGATTTCGACGGTAACTTCAC50.10991426687183997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)