FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_negcon15_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_negcon15_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1060
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCGCACCCGCCTTGAATCGCTGCACCTGAGCATCGACCCGGTGACAGG50.4716981132075472No Hit
CGCCACCCCTGTTCACGCTGACCCTGAACCGCGTGCTGGACAAGGACCTC50.4716981132075472No Hit
GTACGGTAGACGGGCTTATTCGTGTGGAATGATGGCGGGAACCGATCTGA40.37735849056603776No Hit
CATCTGAAGATCCTGATCGATCTTTCGAAATAGGCTTTCCGCCTCCGCAT40.37735849056603776No Hit
GATCAGAGGCGTTAAATCAGAACGAGTGCTGCATAGTTTAGTTGCCAAAG30.2830188679245283No Hit
GTGTGGTCGCTATTTACCCCGACCGTCACGCTGCTGACCGAAGTCGGGCT30.2830188679245283No Hit
GTATAGCTGGGCGACAAGCTTCTCCATCTCCCCAGGACCGACATACAATA30.2830188679245283No Hit
AAACGAATGTGGCGGGCAGTCAGCCCGCGCTTGAGACCTTGTGCAGGTTG30.2830188679245283No Hit
TCTTGCAGGGTCGTCGAGTATTTCCTTGGCGCTCAATTGAATGATCCTGA30.2830188679245283No Hit
CATCCAGCAGCAATAAATCCGTCGACGGATGGACTTCACCGGCGGCAGCG30.2830188679245283No Hit
CATTATGAATTTAATACCTACAGTCATTGAGCAAACAAATCGTGGGGAAA30.2830188679245283No Hit
CGTGCACTCATTGATGAGCCGCTCAATGCCGCTTCCCTTGATCTCCACTT30.2830188679245283No Hit
GCGCAATCACGACGGCCGTCTCGTTGTAGAGCAGCTTGACCGGGCCGATG30.2830188679245283No Hit
CCATCTCGAAGTCCATCAGCGCGCCGACTTGTTCCTCGTCATAGCTCAGC30.2830188679245283No Hit
TGTGCGGTCTAACGCCTGAATTAAGCCGACCCGCGAAGCGGGTTCGGCTT30.2830188679245283No Hit
GAAACACGAGAAGTAAGCATTTACGGTGATACCGTTACAGAGGAAAGAGG30.2830188679245283No Hit
ATATCCCGCTGACCGGTGTCACCGTTGAAGTGACCCACGACGCCGCCCAA30.2830188679245283No Hit
GTGGCGCACATAGCGTTGTACTTCTTCATTACTCAGCGCGTCGATGTCCA30.2830188679245283No Hit
CTAGAACAGGTATAGAAGAAAGAAGAATTGCATCTGATGATGTCAATACA30.2830188679245283No Hit
AACACGATCCGGGCCAGCAACTGCTTCATGCCTTGACCCAGGAACACCCC20.18867924528301888No Hit
ATCGTACATGGCGGGGATCGGTCCGATGGCCGGGATGTCAACGTACCGGT20.18867924528301888No Hit
CCTTTGTATACCCGTAGCCGCCGAAGATTTGGACGGCTTCTGTTGTTACT20.18867924528301888No Hit
TGGTAATCCTCTTCGGACACCGGGGTGAAGCGGACTTGCTGGTCGAGTTC20.18867924528301888No Hit
CGGCTGGATCGCCCAGCCCGTGCTGCAGCTATCCACTGTTCCCACCCTCG20.18867924528301888No Hit
GCTTCAACGATGCCCTTGCCGTGCATTTCCAGTTCGCGGGCGAACTCCAC20.18867924528301888No Hit
TATCGATTCCTCGCAATACGTCAATCGTCTGATGAGCCGCGACTACGACA20.18867924528301888No Hit
TCACGCACCTGCGCCGCGCGCTGGAGCTGCACGACAAATCCGGCGTGAAA20.18867924528301888No Hit
CCACTAGCCAGGCGTCGAACTGACCTGAAAACTCCCCTGCATTAATTAAG20.18867924528301888No Hit
AAATGAGCTGGACCTCACCATCGCCGGACGGCACCAGCAGACAGCCCTTC20.18867924528301888No Hit
GATCAGCGCGGACGTATCAATGCCGAGCGCACGCGCACGCGCGAAGATCG20.18867924528301888No Hit
ATTATGGGTGCGGGCTACGATGCTGCCTCCGAAGACGCTGATCCCGCACC20.18867924528301888No Hit
CTCATGCCCAGGAAAATCGATGATGATTCGTAGCTCTCTCGCCACCGGCG20.18867924528301888No Hit
TTATACGCCAGAGCCTCAATCCCGGCGTCAACATTCGCCCTCGCACGACC20.18867924528301888No Hit
AGATTAGTGGAACGCTCTGGAAAGTGCGGCCATAGTGGGTGATAGCCCCG20.18867924528301888No Hit
TCATATAACCATCCTTTATTAACGAGACTCCCTCTAAAAGAAGCGGTCAA20.18867924528301888No Hit
GTGACCCCTGACAACCCGAAGATCCAGCAGGAAGTGCTGGTCACGGCCGG20.18867924528301888No Hit
ATAATATAATGGTTGTATTTGTGCATATATCGTGCGGTTGTAAGATTCCA20.18867924528301888No Hit
GCTTGAGATCGAAACCTGTCTTTTGGGGAACGGATACGGATTTGGCAACG20.18867924528301888No Hit
TTGTTCGCTTTATCATTGGCATGAAACAAATGGGGGATTAGTAAAGGAGA20.18867924528301888No Hit
TGCCATATGTGGGGACTGACACAACGCCATCGCGGGCAAGCGCGCTCCTA20.18867924528301888No Hit
AGCCGGTGCCGCCGGGCTGTATCCGGCCAACCCCACGCCGCAGTGGACCA20.18867924528301888No Hit
CAGGTGCAGGGCCCGGTGCAATCCGCCACGTGCGATGGGCAAAACTGCCT20.18867924528301888No Hit
GCTTCTGTTGTGCGGGTCACTGCTGCTGCCGATGACGGCGGTTGCGCGCT20.18867924528301888No Hit
AGTTTGTATCATTCCATGTGGGGCGAAGAAAATGTGGGACGTCCATCCAG20.18867924528301888No Hit
TCGCAACTTAGCAAGAGCCTCCACCACAAAGCGTGCTCAAAGCAGGCGGA20.18867924528301888No Hit
CACCAGCGTGATGCAGGGGCGCACCACGCTGGTGATCGCGCATCGCCTGT20.18867924528301888No Hit
CTCGCCGCCGGTCGGGACGGGGCAGCTGTTGCGGGCGTCCTTGGTGGCGG20.18867924528301888No Hit
CCTCGGGCGCAGGTTCGGGTAGCCAGCACGCCATCGGTACCGGTGTCATC20.18867924528301888No Hit
GTATTACAGCCCCGTGGCCAACGACGACGGGATTCCCGCCGGGGTCATGG20.18867924528301888No Hit
GTGACCGGTCCGCTGATCGTCGCGCTGAACAAGACCGATCGCATCGAAGA20.18867924528301888No Hit
GTGAAGGTTCTTCATCGCGCCGGCAATCCCGAAACGGCCAACCGGAATCT20.18867924528301888No Hit
TTATAATATCTTTACAAAGGTAACAACATTGAACATTTTTCTATATGTGT20.18867924528301888No Hit
CTGGAGGGGCGGCGCTTTGCCATCACCGTGGAAGACCTCGGCCTGCGCGC20.18867924528301888No Hit
TCACTGGATATGGTGGCATGGTCATTGTGCGACCGAGCGACGAGCACTAT20.18867924528301888No Hit
CTATTCTACCACGGGCAGATTCCGCGGTTTCAGGCGCCGTCGGCAGGAAG20.18867924528301888No Hit
GCCTTTGCCCGCCATGGCAAGGAAATCGCCGCCGTCATCGTCGAACCCGT20.18867924528301888No Hit
AACAAGCTCACCCCTGAGCAACAGGTGATGGTGAAAAACGCCGCCAAGGC20.18867924528301888No Hit
GTGCAGGGCGACTTCCATGCATCGGCAGTTGGGTCCATGTCGCGTTCGGG20.18867924528301888No Hit
GCGTAATCGTATGCCGCCGTGTAGTAGTCGGCAAACGAACGTTGCCCGCC20.18867924528301888No Hit
GCGCGTTAAGCGTTCCCCTGAAATGACATCCGGAGAAGATTAATGCTTGA20.18867924528301888No Hit
CTATTGGCATGAGCAGCGCTGGCATAGCGCCGACAACCCAGCGCCCGGCG20.18867924528301888No Hit
GCAATGCCGTTCACACCTGTGTGTAAACTTCGGCTGTTCATATCGTATTA20.18867924528301888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)