FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_negcon09_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_negcon09_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4460
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACTATT80.17937219730941703No Hit
CCTGGTGGAGAATCACTTGGACACCGATTTATTGCGCAAGCTGTGCGGGG70.1569506726457399No Hit
GAGTAATAGTCTTCCGACATTTGATAGTTGCGGTACGCCTTGGGGCGGCG60.13452914798206278No Hit
TCTCTACATTGATGCCAACACCGATATAAATGGCGCTGCGATCTGCTTCA60.13452914798206278No Hit
CATGGGAACGATGCAGTGGGTCGGATGGCTGCTGTTGGCGGTATTCGCCG60.13452914798206278No Hit
CGGAAAAACGAGGGGCTGGGAGTCAGAAACAGAGCGCTTTGAGCGCAGGC50.11210762331838565No Hit
AGCCTGTTCGGCAGCGGCGCCAGCCAATTTGCCGGCGTGCTGGGCCTGCG50.11210762331838565No Hit
CACTATAGCTTCAGTTTCCAGCTTCCAGGAAACTGGATGAGGTATTTGCA50.11210762331838565No Hit
ACGCATCAGGATGTGCGGCAGACCAGCGGTACCGAACATCAGCGCCATGC50.11210762331838565No Hit
GATACAATCACGATTCGATGATGTGCTTTGAATTGAGCCAATAAAGCAGC50.11210762331838565No Hit
CGGTGGAGGCATCGGTGGGATACTACCCTGGCTGTATTGACCTTCTAACC50.11210762331838565No Hit
GGTAAAAACCGCGTGCATCATCCGTTTGCAACATGCCGGCCACGCAGCAT50.11210762331838565No Hit
CTTGTAGTTGGTGCCGAAGGTCACCGAACCGTCGAAGGCGCCGATCGGCC50.11210762331838565No Hit
GTTCATGTTTAAACGCATTGTTTTGCTCAAAAGCCCTGACGCGGCCGCCG50.11210762331838565No Hit
ACGTCATCGTCATGTTGCCCGACGTCGATAGCTACGCACCGCACATACGG50.11210762331838565No Hit
GATCTAGGTGATCTGCCCCAGTTCCGCCATGCTGATTTCTCGCATTTTGA50.11210762331838565No Hit
GCTCAGCAGTGTACCGATGGGATAGGATTCGAGCAGGTTGTTCAGCACTT50.11210762331838565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)