FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_negcon07_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_negcon07_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4989
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCACTAGCTTGAAAGGAGTCGGGGGCGCGTTAGACGATGCGCTCTCGC80.16035277610743637No Hit
TTCGTATACAGATCCACACAGCACATCCGTTCCACAAGGACGCACTTTTT80.16035277610743637No Hit
ATCCAGTCCGTCGCCGATGCGCTGCAGTACATCAGCTACTACCATCCGGT80.16035277610743637No Hit
GGGCAGGGCGGGGATGTGTTCGTGCTGGACATGGGCGAGCCGGTGCGCAT60.12026458208057728No Hit
GTGAAGATCAGTGCGGCCGACCGCAAGATCATGGCCGCCGTGCCCGACGA60.12026458208057728No Hit
CCCAGGCGCTGACCGCCCCGCCCGGATAGAGCGCCAGGCTGCTGGAGGCA60.12026458208057728No Hit
CCCCTTACACTCCTATCCGAGCCAAGGTAACAATGAGCCTGTTTTCCGCT60.12026458208057728No Hit
CCAGTAAATGACCCGGCGCCCGGCCAGCACCTGCTGCGTGGGTGCCTTGC60.12026458208057728No Hit
GCTCAATGGTAGCTAACAACGGCTCAAGAGCAGGGTGTTGCGGTGCAGCA60.12026458208057728No Hit
CTTTACCGAAGCGGACGTCGACGGCTCCTGGCAGCCGTATCACCCGGTCG60.12026458208057728No Hit
CGTGACCACACACCAGGCGAGCACAGCATCGCGACGATGGCCCGTGTCGA60.12026458208057728No Hit
GGCTCATGCCGCGTAGCTCCAGCTCGGCCATGCCCAGAAAAGCCAGGCTA50.10022048506714773No Hit
CTCATCAGCACGTCGTCGATCAGCACCAGGTGCTGGCCTTCGATCTCAAA50.10022048506714773No Hit
TGGTGGTGTCGTAGATGGCGGCATCGCGCGAGAAGGCGCTGGTCGCCTTG50.10022048506714773No Hit
AGCATGCTCCGCGCGGCCGAAAACGGCTTTGTCACCCCTTTGCACGATTA50.10022048506714773No Hit
TGAAAAAGCTTGGCGTGGAGTCACGCAGTGCGATCGGGCTTGCCGCGCTG50.10022048506714773No Hit
GTTCAATGCGCTCATCCACCTCGCGCTGCTTGATGGCAGCCTGCAACAGG50.10022048506714773No Hit
CAGCAGCAGGGTGGCGCCGTTGCTCAGGATCGGGGTTACGGCGTAGTCGA50.10022048506714773No Hit
TCGCTATCCCGCTTTATAAGCAGCGTGCATTGCTAAAAAAATATTGGTGG50.10022048506714773No Hit
CCAGGGAAGTGCGGGCACGCCGTGTGACGTGTAGGTCCAGCAGTACCAGG50.10022048506714773No Hit
CAACCGCAGCTTGCGGAGCACATGCTTGCGCTGTGGCGTGCACGTCTCGG50.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)