FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_negcon06_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_negcon06_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4618
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACACAGATGCTGCAGTCCACGCAGGCACCCAGACCCTGCGCTTCGAAA80.17323516673884798No Hit
CGCTTGCGGAACGCCTGCTCCAGCCAATCGGCATTCTTGCGAACATCCTC70.151580770896492No Hit
GAAGGGAACATACATCAAAGTGTTAAACAACAAATTTTTAATTGAACTTG70.151580770896492No Hit
AAGATGTGCAGGATGTCATCACTGCGGTGCTGTCTATTTTAAAAGAACAG70.151580770896492No Hit
CATCAGAGCGGTCGTGACGACGACCGACAACGTCAAGAGGCCGAGGGAGC60.12992637505413598No Hit
CGATAGAGCATCGAGGCCACGGCATCCACACGCAGGCCGTCGATATGGAA60.12992637505413598No Hit
GTATCACGGCGGCGTCAGCATGCCCTCCAGCGTGGAGAACCTGGCGTATG60.12992637505413598No Hit
ACCAACCCGGCCCAGGCCGCTGAAGGCACCATCCGCAAGAGCTTCGCGCT60.12992637505413598No Hit
GTGCAGCAGCGCGGCGTACATGAGCGGCAGGCCGAACAGCTGCGTGATGC50.10827197921178No Hit
TCCCATCACTGTCCACGAAGTCAATGAAGTTAAATACCGTCAAGTAGATC50.10827197921178No Hit
GAGGCGCGGCGTTTGCGCGAAATCAGGCGGCGCTGAGATGTGCGTCGGGA50.10827197921178No Hit
GCGTCTACCTGGACATCAGCCACCAGAGCCCCGCGTTCCTCAAGGAACAC50.10827197921178No Hit
ATATATATCGCAACCACCAAGGATTTGTGCATTGCTAAATATCAACTTCA50.10827197921178No Hit
GAGTTAAACTTGCATTCCCGAAGATGATCACAAGGCCCCGATGCTGAATT50.10827197921178No Hit
GCCGTGGTCCACTCGCAGGAGGCTGCATCCCTGTCGTTGATATTGCGGAA50.10827197921178No Hit
AATGGTTATAGTGCGGAACCGCGCGGCGGGCACTCCGCCAAGTCGACTAG50.10827197921178No Hit
ACCTATACCGCGGCTGGCGCCGGTAATCAGGATGACAGGGTTCATAAGGT50.10827197921178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)