FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_Tr3_NCI3a_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_Tr3_NCI3a_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4396
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTAAAGATCACCACTCCCGTCGTGGTCTGATCCGCATGGTTAACCAGC80.18198362147406735No Hit
TCCATGCATGGCGTGTGGGCGTCGCGCGATGGCCAGCGTGCCGTGCTGGT70.15923566878980894No Hit
TTCTTGACCGGAGCGATGGAGAACTCGCTGAACAGCGGCGCAGACCACGA70.15923566878980894No Hit
CTTCCAGGATGGGGCGACCCGCTGTGCCGGAGGGTTCGCCGTCATCGGAC70.15923566878980894No Hit
GCGGTGGAGCAAGCGCTCGGCCCCGTCGACATGCTGGTCAACAACGCGGG70.15923566878980894No Hit
CGACGCACCGTTGCGCGGTCGCGCGTCGAAAACCTCACCAGCCATGGATG70.15923566878980894No Hit
GGCCCGTCCACGAATACAGTTTCTGCACGTTGCCGTTGCGCACGTCGGTG70.15923566878980894No Hit
CTTGTAATAACCGTGTTCAGGATCGGCGAGGCAGAGCGAAAAATAGTCGG60.13648771610555052No Hit
GCCCCTTTCGTCGTTGAATCCCAACTGACGTGCATGCGAGCATCCGGCCC60.13648771610555052No Hit
GTAGTGCGGGATCGTGAAGGCCACTTTCTCGCGGCGTTCGCGGTTGTTGG60.13648771610555052No Hit
CGGTCAACTGCGCGCCTCCCGAGCTGCTGGGCCCACGCTTCCTGCCGATG60.13648771610555052No Hit
GCAGCAGGGTCACCTCGCGGGCAATCACGCCCAGCTTGGGGCGGCCGGGG60.13648771610555052No Hit
GCGAAGCAGGCCATACGGTGCTTGCCGCCTCGCCGCGCAACGGCGTGAAT50.11373976342129208No Hit
ATGACATCGTGCGTGAATGCGATCGTCAGCGTCCTGACGCGCCATATACG50.11373976342129208No Hit
ATCGATTGTCGAGGCGCTCGCCACCGGCGATGCCGCCCCCGCCGTCAACA50.11373976342129208No Hit
GCGTGCATCAAGGCGATGCCCGGTTGCCAGATGCCCATCGCGCTGGTGTG50.11373976342129208No Hit
CCAGAGATCGGCCAGAGACGTGCAGCGGTCCCTCAGGACCGTGTGACGCA50.11373976342129208No Hit
CCGCACACATGGGACTGAAGGGACCGGTTGTCACTACCAAACCGTCGCTG50.11373976342129208No Hit
ATCTTGGCAATGTCCGCCTTGGCTTTGAAGACCTGCATGCGCTTGCGAAC50.11373976342129208No Hit
AGACGTTGGACCTCGCGCTGACGCTCAACCTGCTGCTCGGTCTTTGTCTG50.11373976342129208No Hit
GGGTTCAACACCCCCGACCAGAGCCAGGACCCGGCCAAGCTCCAGGTCAA50.11373976342129208No Hit
GAAAAAGCCTGCTGAAAAGCTCTGGAACAAGGTCCCGGAAGGGACCACCT50.11373976342129208No Hit
ATCCGGTCCCAGACGATGAAGATGCCGGCGTAGTTCTTGTCGAGGTACTC50.11373976342129208No Hit
GTCAATGGTTGCGCCGAAAACGATGCGACCGTCGGCATCCAGCGAAGCCG50.11373976342129208No Hit
ATCTTCGAGAACACTATGGCGCTCACCTCAAGCGCATTCTGGTTCGCACT50.11373976342129208No Hit
GCACTCGGCCACGGCGCAGTTCTTCATCAACACGGTCGACAACGACTTCC50.11373976342129208No Hit
ACCCAGGCCAGCCTCATCCGGTTCATCCTTCTGGGACTGGGCCTCATGCT50.11373976342129208No Hit
CTCTACCTGCCGGACGGGACGACCGTCGTCGACTCGTACGAGTGGTCGAC50.11373976342129208No Hit
CATCGACGCCGTCGTGCACTCGGCGGGCGTCGTGGCGTACGGCCGAACGG50.11373976342129208No Hit
GCCTTCGGGGGCCAGGACCGGGCCATCGACCTGTCGACGCTGACCACCGA50.11373976342129208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)