FastQCFastQC Report
Fri 6 May 2022
H3N72DRX2_n01_124b_KD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N72DRX2_n01_124b_KD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATGGGTGCGCTGACCAGTGACGGCGATGGCGCCAGTCGCCTGTCCA80.15664773839827686No Hit
CATCATCAAAGTCTGCATCAATCTTGTTCAGCCGTTCCATGACCTCGTCG80.15664773839827686No Hit
CCTTGGAGATGCCGTGGGCAATGTAATTGACCACATCGATGCGGGCCACG80.15664773839827686No Hit
TTATTGGCTACCTCATAACGGTTCTTACCATCATAACGGGCAATTGAAGA70.13706677109849227No Hit
CTGGTATACAGAGTGCGCTGGTCAGTCAAGGGAGTATTTCCCTGGTCGGT70.13706677109849227No Hit
CGCATACAGGTTCGGTAGCACCGTCTGGCGCAGCTGGGTCAAGACCTGAT60.11748580379870766No Hit
GTTGGCAAAGCTGCCTTGCAGGTGCTCGGCCCTGAACGCCAACGTCTGTG60.11748580379870766No Hit
GCACTCAGGAATGCACTGCCGGCGGGGGTCAGTTTGACCCCGCGCTTATT60.11748580379870766No Hit
ATTGGAACGATTACCCGAAGCAAAAGGTTGTTGCCACAGGCTATACAGCA60.11748580379870766No Hit
ATAAATAGCATCGCAGATAATGAAGCATTGCGGATTGCCTCTTCTTTCTG60.11748580379870766No Hit
AAAAAGACCCAAACACCCATTGACCTCATCAGCACGATCCCCTATAATTC60.11748580379870766No Hit
CAATGCAATCAGTGTAAATAAAAAGGGCAAAAAATTAAAAACAGTTCTAG60.11748580379870766No Hit
GGCGTTGACTATTCAAATTTATGTTGTCAAAAGAGGAGATACTTTAAACG60.11748580379870766No Hit
CCATTTCAGCTTGCATGTTTTCATCAATTGTTGTTTGAACTTGAAGAGGA60.11748580379870766No Hit
ATTCTGCCCGGCGCTCATTGTATTTGGAATCAGCCAGTGTCCGTTTTTTG60.11748580379870766No Hit
GATTTATTAGAGAATGAAAAATTCGCGGTCAATGCATCTAGGCTGAAGCA60.11748580379870766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)