FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16219383
Sequences flagged as poor quality0
Sequence length37
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260168716.040604010645783No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT1091890.6732007006678368Illumina Single End PCR Primer 1 (100% over 37bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG668300.41203786851817975No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGA454210.2800414787664858Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAGATCGG441300.27208186649270194Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACTGAAGATCATACTTTATCTAAAAAGATAAAAA402180.24796257662822319No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGAAGATCG315730.1946621520683Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275890.17009894889343202No Hit
ATAACTGAAGATCATACTTTATCTAAAAACATAAAAA270490.16676959906551317No Hit
ATAACTGAAGATCATCCTTTATCTAAAAACATAAAAA259800.1601787194987627No Hit
ATAACTGAAGATCATCCTTTATCTAAAAAGATAAAAA250750.15459897580567644No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212910.1312688651596673No Hit
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATTAGAT193090.11904891819867623Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATAACTGAAGATCATACTTTAGCTAAAAAGATAAAAA192900.11893177440843465No Hit
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAG184470.11373428939929467Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTGA1439900.030.5118582
ATAACTG1454500.030.4256571
ACTGAAG1412800.030.3804864
GATCATC636750.030.23196210
AGATCAT1054850.030.2251959
CTGAAGA1450850.030.2167745
ATCATCC604550.029.9763511
GCACACG320750.029.86471718
AACTGAA1463600.029.853223
TGAAGAT1489700.029.8254176
AAGATCA1203200.029.765118
GTGACTG392300.029.5619951
TGACTGG400700.029.5566642
AGAGCAC345200.029.45717215
GGAGTTC414450.029.4130737
GATCCTC137400.029.39925210
GCACGCG35750.029.39613518
TCATACT307700.029.33962612
AGCACAC313750.029.33179717
GATCATA453750.029.2419110
Produced by FastQC (version 0.11.5)