FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2154-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2154-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18837397
Sequences flagged as poor quality0
Sequence length37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT1320830.7011743713847514Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG283280.15038171144346535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA178800.026.43639228
AGTGTAG180300.026.25929329
GTGTAGA186750.025.40989530
AGAGTGT188700.025.16487527
GCACACG43100.024.09745418
CGTGTAG202200.023.95908416
TGTAGAT196450.023.81571631
AAGAGTG201050.023.79635626
GTGTAGG202050.023.7773817
GTCGTGT210000.023.59361814
GTAGGGA203850.023.5723419
GCGTCGT214100.023.409912
TGTAGGG206100.023.3312218
AGCGTCG216150.023.20203811
CGTCGTG216100.023.20023213
GAGCACA70550.022.94060116
AGAGCAC74800.022.52858415
AGCACAC61850.022.48298817
AGAGCGT228850.022.0468259
AAGAGCG230000.022.0172848
Produced by FastQC (version 0.11.5)