FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2153-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2153-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20825621
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT869270.4174041196658674Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG503900.24196157223835008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG137300.022.42837529
GCACACG30700.020.55079818
CGTGTAG162600.020.41219316
GAGTGTA150600.020.37561428
GTCGTGT173050.020.0486514
GCGTCGT177500.019.90448812
TGTAGGG162650.019.72826818
AGCGTCG180500.019.63369211
GTGTAGG166550.019.47190917
GTAGGGA164350.019.45710419
AGAGTGT160550.019.28687727
TCGTGTA180400.019.24895515
GTGTAGA162550.019.1157630
CGTCGTG187300.018.87936213
TGTAGAT166500.018.680731
AAGAGCG195700.018.3516258
AGAGCAC61150.018.15148515
AGCACAC48650.018.13096217
AGAGCGT197250.018.1211519
GAGCGTC196150.018.1143610
Produced by FastQC (version 0.11.5)