FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2152-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2152-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21226848
Sequences flagged as poor quality0
Sequence length37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2215201.0435840497844993Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307970.14508512992602576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA282100.026.94727528
AGTGTAG283100.026.87927229
GTGTAGA288250.026.38753730
AGAGTGT293450.026.00038527
TGTAGAT295750.025.43004231
AAGAGTG304500.025.1943426
GTAGGGA321100.024.1709119
CGTGTAG323800.024.11507816
GTGTAGG322800.024.05062717
TGTAGGG323700.024.00189618
GCACACG46700.023.93291518
GTCGTGT334950.023.90507314
GCGTCGT341200.023.68078612
CGTCGTG342700.023.57713313
AGCGTCG345750.023.4586611
GGGAAAG330300.023.2795422
GAGCGTC357900.022.71372810
TCGTGTA354250.022.66390615
AGAGCGT363500.022.4042859
GAGCACA71050.022.32056216
Produced by FastQC (version 0.11.5)