FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2151-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2151-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19919236
Sequences flagged as poor quality0
Sequence length37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT1560880.7836043510905739Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338730.1700517027861912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG202450.026.4106129
GAGTGTA202100.026.31851228
GTGTAGA212200.025.22576130
AGAGTGT214150.024.88844527
GCACACG44700.024.76037418
AAGAGTG227400.023.58855226
TGTAGAT223750.023.58444831
CGTGTAG234900.023.42809316
GTCGTGT242300.023.39105814
GTGTAGG235250.023.29398317
GTAGGGA236600.023.0870319
GCGTCGT247200.023.07204212
TGTAGGG237100.023.04592318
GAGCACA74600.023.00384316
CGTCGTG249700.022.87823713
AGCGTCG251900.022.73381411
AGAGCAC78900.022.67376715
AGCACAC65350.022.46406617
TCGTGTA263750.021.64144115
GAGCGTC265350.021.63352810
Produced by FastQC (version 0.11.5)