FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2150-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2150-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18206717
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT3506441.9259045988356933Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG708510.3891475876732746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG457750.026.89325529
TGTAGAT452050.026.87111531
GTGTAGA465900.026.35504730
GAGTGTA469550.026.23384528
AGAGTGT484750.025.52982327
AAGAGTG501900.024.79018226
GCACACG28750.022.32175618
AAAGAGT563750.022.29327225
GTGACTG61100.021.7886711
GGGAAAG582150.021.49570722
TAGGGAA590000.021.39476420
TGTAGGG588450.021.37427118
GAAAGAG591200.021.3735824
GTAGGGA587500.021.37341719
CGTGTAG596750.021.19769716
GGAAAGA607350.021.08356323
GTGTAGG597500.021.04643617
GTCGTGT624050.020.779914
TCGTGTA627150.020.68454415
TGACTGG63450.020.5667862
Produced by FastQC (version 0.11.5)