FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2149-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2149-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21920673
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT1088710.49665902137219964Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG505770.23072740513030784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG160650.023.45190829
GAGTGTA177000.021.24195128
CGTGTAG191400.020.7101216
GTCGTGT199850.020.66473614
AGAGTGT183400.020.65293327
TGTAGAT182250.020.4846431
GCGTCGT206250.020.43699512
TGTAGGG192150.020.2816818
AGCGTCG210000.020.2640311
GTGTAGG194500.020.1329617
GTAGGGA193850.020.09503719
GTGTAGA188450.019.99979430
TCGTGTA207950.019.99409115
CGTCGTG216950.019.31462113
AAGAGTG202600.018.97103926
GAGCGTC225800.018.93499410
AAGAGCG229200.018.8682618
AGAGCGT229050.018.718299
TAGGGAA216300.018.27338620
GCACACG21900.017.20050218
Produced by FastQC (version 0.11.5)