FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2148-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2148-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28360694
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2320530.8182204568054647Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG738300.26032508231286583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG337300.024.39384329
TGTAGAT349250.023.3007331
GAGTGTA354150.023.29464528
GTGTAGA366600.022.4561330
AGAGTGT372250.022.2579427
AAGAGTG396350.021.15499726
TGTAGGG416700.020.42656118
CGTGTAG421600.020.37364216
GTAGGGA418800.020.26010519
GTGTAGG423850.020.09373317
GTCGTGT443200.020.03159114
TCGTGTA450700.019.71878415
GCACACG39150.019.67849718
GCGTCGT463600.019.46465712
TAGGGAA453350.019.07447420
AGCGTCG476150.019.03284811
CGTCGTG478050.018.83417913
AAAGAGT459800.018.47186525
GAGCGTC505950.017.92376910
AAGAGCG513350.017.8810528
Produced by FastQC (version 0.11.5)