FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2147-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2147-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19720002
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT9776454.957631343039417Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG797690.40450807256510424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT1106350.029.53295931
GAGTGTA1156900.028.70035428
GTGTAGA1154550.028.68250730
AGTGTAG1160550.028.64080429
AGAGTGT1166000.028.51911527
AAGAGTG1183700.028.1872226
AAAGAGT1257900.026.75760525
GGAAAGA1282600.026.70566623
GAAAGAG1271000.026.61642624
GGGAAAG1265000.026.5262822
GTAGGGA1313200.025.83663419
AGGGAAA1328100.025.76455521
TGTAGGG1319750.025.66488318
TAGGGAA1326800.025.63962620
GTGTAGG1326500.025.55953617
CGTGTAG1335700.025.4462916
GTCGTGT1384150.025.14264114
TCGTGTA1390200.025.05100415
CGTCGTG1398950.024.95317513
GCGTCGT1402450.024.91521812
Produced by FastQC (version 0.11.5)