FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2146-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2146-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762864
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT7442904.440112381750517Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG867560.5175487911850862No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA174800.1042781233564861Illumina Single End PCR Primer 1 (100% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT876100.028.85233931
GTGTAGA938050.027.25805330
AGTGTAG964300.026.59742529
GAGTGTA970400.026.42072928
AGAGTGT987700.026.01491227
AAGAGTG1000500.025.78138426
GTGACTG87250.024.2982371
TGACTGG88100.023.730792
AAAGAGT1102950.023.59339525
GAAAGAG1123650.023.29979324
GGAAAGA1147450.023.16555823
GGGAAAG1140200.022.84530822
TAGGGAA1191050.022.18640720
AGGGAAA1212200.021.93642421
GTAGGGA1206550.021.8107619
TGTAGGG1207750.021.77324918
ACGTGTA38100.021.68658315
GTGTAGG1215050.021.64710417
CGTGTAG1221050.021.62560316
TCGTGTA1258800.021.39350715
Produced by FastQC (version 0.11.5)