FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2114-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2114-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13023302
Sequences flagged as poor quality0
Sequence length37
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT573530.44038754533988383Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254220.19520395058027526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG91050.022.48515529
CGTGTAG100450.021.3742716
GTCGTGT105800.020.9531114
GAGTGTA98500.020.6747628
TGTAGGG102050.020.47622918
GCGTCGT110700.020.40359512
GTAGGGA102200.020.39988719
GTGTAGG103800.020.38575417
TCGTGTA108950.020.26177615
CGTCGTG111800.020.23057613
AGCGTCG113000.020.04302211
AGAGTGT104100.019.65212627
GTGTAGA106300.019.25872630
TGTAGAT103850.019.23546831
GAGCGTC118800.019.01185610
AAGAGCG120850.018.8800248
AGAGCGT122100.018.6615649
TAGGGAA118650.017.94983120
AAGAGTG120150.017.51717226
GATCGGA122550.017.4775052
Produced by FastQC (version 0.11.5)