FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2112-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2112-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17948583
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2785031.5516712377796065Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG708530.3947553965680745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGAT382400.026.65489831
AGTGTAG406950.026.03769329
GTGTAGA400250.025.79170230
GAGTGTA413950.025.60127428
AGAGTGT432800.024.95211627
AAGAGTG453950.024.19544426
GCACACG29300.021.26808518
AAAGAGT513800.021.16642425
GTAGGGA505350.021.026519
TGTAGGG514000.020.93890818
CGTGTAG518700.020.8633816
TAGGGAA511400.020.83394820
AGAGCAC69350.020.67691815
GTGTAGG523300.020.60598217
GGGAAAG539950.020.552722
GTCGTGT543800.020.52212314
GTGACTG55400.020.4776691
TCGTGTA532850.020.46387715
GAAAGAG541850.020.41702324
GGAAAGA553100.020.20971923
Produced by FastQC (version 0.11.5)