FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2104-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2104-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26378991
Sequences flagged as poor quality0
Sequence length37
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT2947261.1172754863899077Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG381960.14479704701366325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA374100.026.89479828
AGTGTAG379750.026.6739729
GTGTAGA389300.026.09078630
AGAGTGT387450.026.07230827
TGTAGAT390650.025.63187231
AAGAGTG404050.025.07786426
GTAGGGA452650.022.85438519
TGTAGGG458350.022.52055218
GTGTAGG462900.022.39079517
CGTGTAG467100.022.25924516
GCACACG49450.022.1939318
GGGAAAG462700.022.14139422
GTCGTGT493750.021.57316414
AAAGAGT478700.021.42987425
GGAAAGA492150.021.26684623
CGTCGTG507550.021.15471313
GCGTCGT510100.021.1250212
AGCACAC65400.021.00130517
TCGTGTA510700.020.9177115
AGCGTCG520000.020.8121111
Produced by FastQC (version 0.11.5)