FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2103-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2103-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23545720
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT3327221.4130890879531397Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG405850.17236678258299173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG443250.026.21630929
TGTAGAT442550.025.88235331
GAGTGTA449000.025.85663628
AGAGTGT463950.025.0674627
GTGTAGA468500.024.80268330
AAGAGTG485400.024.14815326
AAAGAGT527200.022.49839225
GTAGGGA535950.022.16865319
TGTAGGG538600.022.03470818
TAGGGAA544000.021.96448520
CGTGTAG552100.021.6205116
GTGTAGG551200.021.57969317
GGGAAAG552300.021.31660322
TCGTGTA582900.021.15640415
GTCGTGT585500.021.03346414
GGAAAGA585900.020.66532323
GAAAGAG580700.020.519224
GCGTCGT610100.020.33528512
CGTCGTG610250.020.3252513
AGGGAAA598400.020.12528821
Produced by FastQC (version 0.11.5)