FastQCFastQC Report
Mon 13 Jul 2020
H3N5MBGXG_n02_DS2100-1.dksk.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3N5MBGXG_n02_DS2100-1.dksk.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456434
Sequences flagged as poor quality0
Sequence length37
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT4785691.7430122207421401Illumina Single End PCR Primer 1 (100% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337640.1229729978772917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA613850.027.13614528
AGTGTAG616800.027.0439529
TGTAGAT611800.026.8741731
GTGTAGA625000.026.66365630
AGAGTGT629450.026.56229427
AAGAGTG653100.025.79036126
GGGAAAG732900.023.8250122
AAAGAGT725500.023.6185725
GTAGGGA759800.023.38970619
GGAAAGA761850.023.3711823
TGTAGGG766300.023.18005618
GTGTAGG774700.023.03781517
CGTGTAG778800.023.03418416
GAAAGAG767650.022.84303724
GCACACG54400.022.59648318
GTCGTGT814550.022.55255114
AGGGAAA800150.022.42331921
CGTCGTG830950.022.41332213
TAGGGAA797800.022.37527720
TCGTGTA826900.022.24192815
Produced by FastQC (version 0.11.5)